In Vitro Modulator Effect of Total Extract from the Endophytic Paenibacillus polymyxa RNC-D in Leishmania (Leishmania) amazonensis and Macrophages
Joint Authors
Neris, Débora Meira
Ortolani, Letícia Gonçalves
Castro, Cynthia Aparecida de
Correia, Ricardo de Oliveira
Rodolpho, Joice Margareth de Almeida
Camillo, Luciana
Nogueira, Camila Tita
Sousa, Cristina Paiva de
Anibal, Fernanda de Freitas
Source
International Journal of Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-27
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Leishmaniases are diseases with high epidemiological relevance and wide geographical distribution.
In Brazil, Leishmania (Leishmania) amazonensis is related to the tegumentary form of leishmaniasis.
The treatment for those diseases is problematic as the available drugs promote adverse effects in patients.
Therefore, it is important to find new therapeutic targets.
In this regard, one alternative is the study of biomolecules produced by endophytic microorganisms.
In this study, the total extract produced by the endophytic Paenibacillus polymyxa RNC-D was used to evaluate the leishmanicidal, nitric oxide, and cytokines production using RAW 264.7 macrophages.
The results showed that, in the leishmanicidal assay with L.
amazonensis, EC50 values at the periods of 24 and 48 hours were 0.624 mg/mL and 0.547 mg/mL, respectively.
Furthermore, the cells treated with the extract presented approximately 25% of infected cells with an average of 3 amastigotes/cell in the periods of 24 and 48 hours.
Regarding the production of cytokines in RAW 264.7 macrophages infected/treated with the extract, a significant increase in TNF-α was observed at the periods of 24 and 48 hours in the treated cells.
The concentrations of IFN-γ and IL-12 showed significant increase in 48 hours.
A significant decrease in IL-4 was observed in all cells treated with the extract in 24 hours.
It was observed in the treated cells that the NO production by RAW 264.7 macrophages increased between 48 and 72 hours.
The endophytic Paenibacillus polymyxa RNC-D extract modulates the mediators of inflammation produced by RAW 264.7 macrophages promoting L.
amazonensis death.
The immunomodulatory effects might be a promising target to develop new immunotherapeutic and antileishmanial drugs.
American Psychological Association (APA)
Neris, Débora Meira& Ortolani, Letícia Gonçalves& Castro, Cynthia Aparecida de& Correia, Ricardo de Oliveira& Rodolpho, Joice Margareth de Almeida& Camillo, Luciana…[et al.]. 2020. In Vitro Modulator Effect of Total Extract from the Endophytic Paenibacillus polymyxa RNC-D in Leishmania (Leishmania) amazonensis and Macrophages. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172527
Modern Language Association (MLA)
Neris, Débora Meira…[et al.]. In Vitro Modulator Effect of Total Extract from the Endophytic Paenibacillus polymyxa RNC-D in Leishmania (Leishmania) amazonensis and Macrophages. International Journal of Microbiology No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1172527
American Medical Association (AMA)
Neris, Débora Meira& Ortolani, Letícia Gonçalves& Castro, Cynthia Aparecida de& Correia, Ricardo de Oliveira& Rodolpho, Joice Margareth de Almeida& Camillo, Luciana…[et al.]. In Vitro Modulator Effect of Total Extract from the Endophytic Paenibacillus polymyxa RNC-D in Leishmania (Leishmania) amazonensis and Macrophages. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172527
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172527