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Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds
Joint Authors
Photolo, Mampolelo M.
Mavumengwana, Vuyo
Sitole, Lungile
Tlou, Matsobane G.
Source
International Journal of Microbiology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This study reports on the isolation and identification of Methylobacterium radiotolerans MAMP 4754 from the seeds of the medicinal plant, Combretum erythrophyllum, for the purposes of investigating antimicrobial and antioxidant activities from this endophyte.
The strain identity was confirmed by 16S rRNA-based phylogeny and Scanning Electron Microscopy (SEM).
Ethyl acetate and chloroform (1 : 1 v/v) extracts from the endophyte were tested for antimicrobial and antioxidant activity on a total of 7 bacterial species (3 Gram-positive and 4 Gram-negative) using the standard Minimum Inhibitory Concentration (MIC) protocol and Quantitative Radical Scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively.
The MICs were recorded at 250 μg/mL for B.
subtilis ATCC 19659, B.
cereus ATCC 1076, E.
coli ATCC1053, and 62.5 μg/mL for K.
oxytoca ATCC 13182 and M.
smegmatis ATCC 21293, while an IC50 of 5.65 μg/mL was recorded with the DPPH assay.
Qualitative phytochemical analysis was positive for alkaloids, flavonoids, and steroids.
Gas chromatography/mass spectrometry (GC/MS) analysis revealed the presence of 9-octadecene, 2,4-dinitrophenyl acetate, and 2(5H)-furanone, which have been previously reported for the targeted activities.
M.
radiotolerans MAMP 4754 tested positive for antimicrobial and antioxidant activity and this is linked to the production of plant-derived secondary metabolites by this strain.
American Psychological Association (APA)
Photolo, Mampolelo M.& Mavumengwana, Vuyo& Sitole, Lungile& Tlou, Matsobane G.. 2020. Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds. International Journal of Microbiology،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172560
Modern Language Association (MLA)
Tlou, Matsobane G.…[et al.]. Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds. International Journal of Microbiology No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1172560
American Medical Association (AMA)
Photolo, Mampolelo M.& Mavumengwana, Vuyo& Sitole, Lungile& Tlou, Matsobane G.. Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds. International Journal of Microbiology. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1172560
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172560