Characterization of Transcription Termination-Associated RNAs: New Insights into their Biogenesis, Tailing, and Expression in Primary Tumors
Joint Authors
Laudadio, Ilaria
Formichetti, Sara
Gioiosa, Silvia
Klironomos, Filippos
Rajewsky, Nikolaus
Macino, Giuseppe
Carissimi, Claudia
Fulci, Valerio
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Next-generation sequencing has uncovered novel classes of small RNAs (sRNAs) in eukaryotes, in addition to the well-known miRNAs, siRNAs, and piRNAs.
In particular, sRNA species arise from transcription start sites (TSSs) and the transcription termination sites (TTSs) of genes.
However, a detailed characterization of these new classes of sRNAs is still lacking.
Here, we present a comprehensive study of sRNAs derived from TTSs of expressed genes (TTSa-RNAs) in human cell lines and primary tissues.
Taking advantage of sRNA-sequencing, we show that TTSa-RNAs are present in the nuclei of human cells, are loaded onto both AGO1 and AGO2, and their biogenesis does not require DICER and AGO2 endonucleolytic activity.
TTSa-RNAs display a strong bias against a G residue in the first position at 5′ end, a known feature of AGO-bound sRNAs, and a peculiar oligoA tail at 3′ end.
AGO-bound TTSa-RNAs derive from genes involved in cell cycle progression regulation and DNA integrity checkpoints.
Finally, we provide evidence that TTSa-RNAs can be detected by sRNA-Seq in primary human tissue, and their expression increases in tumor samples as compared to nontumor tissues, suggesting that in the future, TTSa-RNAs might be explored as biomarker for diagnosis or prognosis of human malignancies.
American Psychological Association (APA)
Laudadio, Ilaria& Formichetti, Sara& Gioiosa, Silvia& Klironomos, Filippos& Rajewsky, Nikolaus& Macino, Giuseppe…[et al.]. 2018. Characterization of Transcription Termination-Associated RNAs: New Insights into their Biogenesis, Tailing, and Expression in Primary Tumors. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1172664
Modern Language Association (MLA)
Laudadio, Ilaria…[et al.]. Characterization of Transcription Termination-Associated RNAs: New Insights into their Biogenesis, Tailing, and Expression in Primary Tumors. International Journal of Genomics No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1172664
American Medical Association (AMA)
Laudadio, Ilaria& Formichetti, Sara& Gioiosa, Silvia& Klironomos, Filippos& Rajewsky, Nikolaus& Macino, Giuseppe…[et al.]. Characterization of Transcription Termination-Associated RNAs: New Insights into their Biogenesis, Tailing, and Expression in Primary Tumors. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1172664
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172664