Astragalus membranaceus and Panax notoginseng, the Novel Renoprotective Compound, Synergistically Protect against Podocyte Injury in Streptozotocin-Induced Diabetic Rats
Joint Authors
Chen, Teng
Xu, Youhua
Zhai, Ruonan
Jian, Guihua
Xie, Ling
Xue, Rui
Gao, Chongting
Wang, Niansong
Gui, Dingkun
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-16
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
This study was aimed at investigating the synergistical protective effects of Astragalus membranaceus (AG) and Panax notoginseng (NG) on podocyte injury in diabetic rats.
Diabetes was induced in rats by a single intraperitoneal injection of streptozotocin at 55 mg/kg.
Diabetic rats were then orally administrated with losartan, AG, NG, and AG plus NG (2 : 1) for 12 weeks.
Albuminuria, biochemical markers, renal histopathology, and podocyte number per glomerulus were measured.
Podocyte apoptosis was determined by triple immunofluorescence labeling including TUNEL assay, WT1, and DAPI.
Renal expression of nephrin, α-dystroglycan, Bax, Bcl-xl, and Nox4 was evaluated by immunohistochemistry, western blot, and RT-PCR.
AG plus NG ameliorated albuminuria, renal histopathology, and podocyte foot process effacement to a greater degree than did AG or NG alone.
The number of podocytes per glomerulus, as well as renal expression of nephrin, α-dystroglycan, and Bcl-xl, was decreased, while podocyte apoptosis, as well as renal expression of Bax and Nox4, was increased in diabetic rats.
All of these abnormalities were partially restored by AG plus NG to a greater degree than did AG or NG alone.
In conclusion, AG and NG synergistically ameliorated diabetic podocyte injury partly through upregulation of nephrin, α-dystroglycan, and Bcl-xl, as well as downregulation of Bax and Nox4.
These findings might provide a novel treatment combination for DN.
American Psychological Association (APA)
Zhai, Ruonan& Jian, Guihua& Chen, Teng& Xie, Ling& Xue, Rui& Gao, Chongting…[et al.]. 2019. Astragalus membranaceus and Panax notoginseng, the Novel Renoprotective Compound, Synergistically Protect against Podocyte Injury in Streptozotocin-Induced Diabetic Rats. Journal of Diabetes Research،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1172716
Modern Language Association (MLA)
Zhai, Ruonan…[et al.]. Astragalus membranaceus and Panax notoginseng, the Novel Renoprotective Compound, Synergistically Protect against Podocyte Injury in Streptozotocin-Induced Diabetic Rats. Journal of Diabetes Research No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1172716
American Medical Association (AMA)
Zhai, Ruonan& Jian, Guihua& Chen, Teng& Xie, Ling& Xue, Rui& Gao, Chongting…[et al.]. Astragalus membranaceus and Panax notoginseng, the Novel Renoprotective Compound, Synergistically Protect against Podocyte Injury in Streptozotocin-Induced Diabetic Rats. Journal of Diabetes Research. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1172716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172716