Genome Analysis of Rhodococcus Sp. DSSKP-R-001: A Highly Effective β-Estradiol-Degrading Bacterium
Joint Authors
Qiu, Qing
Tian, Kejian
Huo, Hongliang
Zhao, Hongyan
Wang, Yu
Zhang, Hongyan
Ma, Shuang
Jin, Shenbao
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-28
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
We screened bacteria that use E2 as its sole source of carbon and energy for growth and identified them as Rhodococcus, and we named them DSSKP-R-001.
For a better understanding of the metabolic potential of the strain, whole genome sequencing of Rhodococcus DSSKP-R-001 and annotation of the functional genes were performed.
The genomic sketches included a predicted protein-coding gene of approximately 5.4 Mbp with G + C content of 68.72% and 5180.
The genome of Rhodococcus strain DSSKP-R-001 consists of three replicons: one chromosome and two plasmids of 5.2, 0.09, and 0.09, respectively.
The results showed that there were ten steroid-degrading enzymes distributed in the whole genome of the strain.
The existence and expression of estradiol-degrading enzymes were verified by PCR and RTPCR.
Finally, comparative genomics was used to compare multiple strains of Rhodococcus.
It was found that Rhodococcus DSSKP-R-001 had the highest similarity to Rhodococcus sp.
P14 and there were 2070 core genes shared with Rhodococcus sp.
P14, Rhodococcus jostii RHA1, Rhodococcus opacus B4, and Rhodococcus equi 103S, showing evolutionary homology.
In summary, this study provides a comprehensive understanding of the role of Rhodococcus DSSKP-R-001 in estradiol-efficient degradation of these assays for Rhodococcus.
DSSKP-R-001 in bioremediation and evolution within Rhodococcus has important meaning.
American Psychological Association (APA)
Zhao, Hongyan& Tian, Kejian& Qiu, Qing& Wang, Yu& Zhang, Hongyan& Ma, Shuang…[et al.]. 2018. Genome Analysis of Rhodococcus Sp. DSSKP-R-001: A Highly Effective β-Estradiol-Degrading Bacterium. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1172717
Modern Language Association (MLA)
Zhao, Hongyan…[et al.]. Genome Analysis of Rhodococcus Sp. DSSKP-R-001: A Highly Effective β-Estradiol-Degrading Bacterium. International Journal of Genomics No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1172717
American Medical Association (AMA)
Zhao, Hongyan& Tian, Kejian& Qiu, Qing& Wang, Yu& Zhang, Hongyan& Ma, Shuang…[et al.]. Genome Analysis of Rhodococcus Sp. DSSKP-R-001: A Highly Effective β-Estradiol-Degrading Bacterium. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1172717
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172717