Transcriptome and Expression Profiling Analysis of Recalcitrant Tea (Camellia sinensis L.) Seeds Sensitive to Dehydration
Joint Authors
Jiang, Changjun
Jin, Xiaofang
Liu, Dandan
Ma, Linlong
Gong, Ziming
Cao, Dan
Liu, Yanli
Li, Yeyun
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The tea plant (Camellia sinensis (L.) O.
Kuntze) is an economically important woody perennial nonalcoholic health beverage crop.
Tea seeds are categorized as recalcitrant and are sensitive to dehydration treatment.
However, the molecular basis of this phenomenon has not been investigated.
Thus, we analyzed the genome-wide expression profiles of three dehydration stages using RNA-Seq and digital gene expression (DGE) technologies.
We performed de novo assembly and obtained a total of 91,925 nonredundant unigenes, of which 58,472 were extensively annotated.
By a hierarchical clustering of differentially expressed genes (DEGs), we found that 8929 DEGs were downregulated and 5875 DEGs were upregulated during dehydration treatment.
A series of genes related to ABA biosynthesis and signal transduction, transcription factor, antioxidant enzyme, LEA protein, and proline metabolism that have been reported to function in dehydration process were found to be downregulated.
Additionally, the expression profiles of 12 selected genes related to tea seed dehydration treatment were confirmed by qRT-PCR analysis.
To our knowledge, this is the first genome-wide study elucidating the possible molecular mechanisms of sensitivity of recalcitrant tea seeds to dehydration.
The results obtained in this study contribute to the preservation of tea seeds as genetic resources and can also be used to explore the mechanism of dehydration sensitivity of other recalcitrant seeds.
American Psychological Association (APA)
Jin, Xiaofang& Liu, Dandan& Ma, Linlong& Gong, Ziming& Cao, Dan& Liu, Yanli…[et al.]. 2018. Transcriptome and Expression Profiling Analysis of Recalcitrant Tea (Camellia sinensis L.) Seeds Sensitive to Dehydration. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1172788
Modern Language Association (MLA)
Jin, Xiaofang…[et al.]. Transcriptome and Expression Profiling Analysis of Recalcitrant Tea (Camellia sinensis L.) Seeds Sensitive to Dehydration. International Journal of Genomics No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1172788
American Medical Association (AMA)
Jin, Xiaofang& Liu, Dandan& Ma, Linlong& Gong, Ziming& Cao, Dan& Liu, Yanli…[et al.]. Transcriptome and Expression Profiling Analysis of Recalcitrant Tea (Camellia sinensis L.) Seeds Sensitive to Dehydration. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1172788
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172788