Autophagy and mTOR Pathways Mediate the Potential Renoprotective Effects of Vitamin D on Diabetic Nephropathy
Joint Authors
Samaka, Rehab M.
Khodir, Suzan A.
Ameen, Omnia
Source
International Journal of Nephrology
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Introduction.
Not only is diabetic nephropathy (DN) the most common cause of end-stage renal disease worldwide, but it also increases the risk of mortality up to fourteen times compared to normoalbuminuric diabetic patients.
Aim.
The aim of the current study was the evaluation of the renoprotective effects of vitamin D in DN and the possible interplay between autophagy and mTOR pathways.
Materials and Methods.
Fifty male Wistar albino rats were divided (10/group) into control, DN group, insulin-treated DN group, vitamin D-treated DN group, and combined insulin and vitamin D-treated DN group.
Assessments of systolic blood pressure, albuminuria, creatinine clearance, serum glucose, insulin, urea, creatinine, inflammatory cytokines, oxidative stress markers, and rat kidney gene expression of mTOR were performed.
Histopathological and immunohistochemical assessments of autophagy marker LC3 in rat kidneys were also performed.
Results.
DN was associated with significant increases in SBP, urinary albumin, serum glucose, urea, creatinine, inflammatory cytokines, MDA, and mTOR gene expression (P<0.05).
However, there was significant decrease in creatinine clearance, serum insulin, GSH, and H score value of LC3 when compared with control group (P<0.05).
The combination of insulin and vitamin D treatment significantly restored DN changes when compared with the other treated groups, except in oxidative stress markers where there was an insignificant difference between the combination-treated and insulin-treated groups (P>0.05).
Conclusion.
It has been concluded that vitamin D is a potent adjuvant therapy in treatment of DN via downregulation of mTOR gene expression, stimulation of autophagy, and antioxidant, anti-inflammatory, and hypotensive effects.
American Psychological Association (APA)
Khodir, Suzan A.& Samaka, Rehab M.& Ameen, Omnia. 2020. Autophagy and mTOR Pathways Mediate the Potential Renoprotective Effects of Vitamin D on Diabetic Nephropathy. International Journal of Nephrology،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1172794
Modern Language Association (MLA)
Khodir, Suzan A.…[et al.]. Autophagy and mTOR Pathways Mediate the Potential Renoprotective Effects of Vitamin D on Diabetic Nephropathy. International Journal of Nephrology No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1172794
American Medical Association (AMA)
Khodir, Suzan A.& Samaka, Rehab M.& Ameen, Omnia. Autophagy and mTOR Pathways Mediate the Potential Renoprotective Effects of Vitamin D on Diabetic Nephropathy. International Journal of Nephrology. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1172794
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172794