Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development
Joint Authors
Ropka-Molik, Katarzyna
Piórkowska, Katarzyna
Żukowski, Kacper
Połtowicz, Katarzyna
Nowak, Joanna
Wojtysiak, Dorota
Derebecka, Natalia
Wesoły, Joanna
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The hypothalamus plays an overarching role that is reflected in the physiological processes observed in the entire organism.
The hypothalamus regulates selected metabolic processes and activities of the autonomic nervous system.
The avian hypothalamus due to the structural complexity is not well described and has a slightly different function than the mammalian hypothalamus that is the subject of numerous studies.
The present study evaluated activities of hypothalamic genes in fast-growing chickens during development (at the 1st day and 3rd and 6th weeks after hatching).
The hypothalamic transcriptomes for 3- and 6-week-old cockerels were analysed using an RNA sequencing method in next-generation sequencing (NGS) technology.
The differentially expressed gene analysis was conducted using DESeq2 software.
In younger 22-day-old cockerels, 389 genes showed higher expression (fold change > 1.5) than that in 45-day-old birds.
These genes played a role in several biological processes because they encoded proteins involved in integrin signalling, regulation of hormone levels, camera-type eye development, and blood vessel development.
Moreover, surprisingly in the hypothalamus of 3-week-old cockerels, transcripts were identified for proteins involved in both anorexigenic (POMC, NMU) and orexigenic (PMCH, ALDH1A1, LPL, and GHRH) pathways.
The RNA-seq results were confirmed by qPCR methods.
In summary, the intensive growth of 3-week-old chickens was reflected in hypothalamic activities because the genes associated with the somatotropin axis and regulation of satiety centre showed increased expression.
American Psychological Association (APA)
Piórkowska, Katarzyna& Żukowski, Kacper& Połtowicz, Katarzyna& Nowak, Joanna& Wojtysiak, Dorota& Derebecka, Natalia…[et al.]. 2018. Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1172795
Modern Language Association (MLA)
Piórkowska, Katarzyna…[et al.]. Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development. International Journal of Genomics No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1172795
American Medical Association (AMA)
Piórkowska, Katarzyna& Żukowski, Kacper& Połtowicz, Katarzyna& Nowak, Joanna& Wojtysiak, Dorota& Derebecka, Natalia…[et al.]. Transcriptomic Changes in Broiler Chicken Hypothalamus during Growth and Development. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1172795
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172795