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Transcriptome Analysis of the Thymus in Short-Term Calorie-Restricted Mice Using RNA-seq
Joint Authors
Ozdemir Ozgenturk, Nehir
Omeroglu Ulu, Zehra
Ulu, Salih
Dogan, Soner
Guvenc Tuna, Bilge
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Calorie restriction (CR), which is a factor that expands lifespan and an important player in immune response, is an effective protective method against cancer development.
Thymus, which plays a critical role in the development of the immune system, reacts to nutrition deficiency quickly.
RNA-seq-based transcriptome sequencing was performed to thymus tissues of MMTV-TGF-α mice subjected to ad libitum (AL), chronic calorie restriction (CCR), and intermittent calorie restriction (ICR) diets in this study.
Three cDNA libraries were sequenced using Illumina HiSeq™ 4000 to produce 100 base pair-end reads.
On average, 105 million clean reads were mapped and in total 6091 significantly differentially expressed genes (DEGs) were identified (p<0.05).
These DEGs were clustered into Gene Ontology (GO) categories.
The expression pattern revealed by RNA-seq was validated by quantitative real-time PCR (qPCR) analysis of four important genes, which are leptin, ghrelin, Igf1, and adinopectin.
RNA-seq data has been deposited in NCBI Gene Expression Omnibus (GEO) database (GSE95371).
We report the use of RNA sequencing to find DEGs that are affected by different feeding regimes in the thymus.
American Psychological Association (APA)
Omeroglu Ulu, Zehra& Ulu, Salih& Dogan, Soner& Guvenc Tuna, Bilge& Ozdemir Ozgenturk, Nehir. 2018. Transcriptome Analysis of the Thymus in Short-Term Calorie-Restricted Mice Using RNA-seq. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1172843
Modern Language Association (MLA)
Omeroglu Ulu, Zehra…[et al.]. Transcriptome Analysis of the Thymus in Short-Term Calorie-Restricted Mice Using RNA-seq. International Journal of Genomics No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1172843
American Medical Association (AMA)
Omeroglu Ulu, Zehra& Ulu, Salih& Dogan, Soner& Guvenc Tuna, Bilge& Ozdemir Ozgenturk, Nehir. Transcriptome Analysis of the Thymus in Short-Term Calorie-Restricted Mice Using RNA-seq. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1172843
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172843