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Effect of Baoshenfang Formula on Podocyte Injury via Inhibiting the NOX-4ROSp38 Pathway in Diabetic Nephropathy
Joint Authors
Cui, Fangqiang
Gao, Yanbin
Zhao, Wenjing
Liu, Wei Jing
Shen, Zi-Long
Tang, Long
Wang, Yue-fen
Meng, Yuan
Shen, Cun
Liu, Zhi-qiang
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-16
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Diabetic nephropathy (DN) is a serious kidney-related complication of type 1 and type 2 diabetes.
The Chinese herbal formula Baoshenfang (BSF) shows therapeutic potential in attenuating oxidative stress and apoptosis in podocytes in DN.
This study evaluated the effects of BSF on podocyte injury in vivo and in vitro and explored the possible involvement of the nicotinamide adenine dinucleotide phosphate-oxidase-4/reactive oxygen species- (NOX-4/ROS-) activated p38 pathway.
In the identified compounds by mass spectrometry, some active constituents of BSF were reported to show antioxidative activity.
In addition, we found that BSF significantly decreased 24-hour urinary protein, serum creatinine, and blood urea nitrogen in DN patients.
BSF treatment increased the nephrin expression, alleviated oxidative cellular damage, and inhibited Bcl-2 family-associated podocyte apoptosis in high-glucose cultured podocytes and/or in diabetic rats.
More importantly, BSF also decreased phospho-p38, while high glucose-mediated apoptosis was blocked by p38 mitogen-activated protein kinase inhibitor in cultured podocytes, indicating that the antiapoptotic effect of BSF is p38 pathway-dependent.
High glucose-induced upexpression of NOX-4 was normalized by BSF, and NOX-4 siRNAs inhibited the phosphorylation of p38, suggesting that the activated p38 pathway is at least partially mediated by NOX-4.
In conclusion, BSF can decrease proteinuria and protect podocytes from injury in DN, in part through inhibiting the NOX-4/ROS/p38 pathway.
American Psychological Association (APA)
Cui, Fangqiang& Tang, Long& Gao, Yanbin& Wang, Yue-fen& Meng, Yuan& Shen, Cun…[et al.]. 2019. Effect of Baoshenfang Formula on Podocyte Injury via Inhibiting the NOX-4ROSp38 Pathway in Diabetic Nephropathy. Journal of Diabetes Research،Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1172867
Modern Language Association (MLA)
Cui, Fangqiang…[et al.]. Effect of Baoshenfang Formula on Podocyte Injury via Inhibiting the NOX-4ROSp38 Pathway in Diabetic Nephropathy. Journal of Diabetes Research No. 2019 (2019), pp.1-16.
https://search.emarefa.net/detail/BIM-1172867
American Medical Association (AMA)
Cui, Fangqiang& Tang, Long& Gao, Yanbin& Wang, Yue-fen& Meng, Yuan& Shen, Cun…[et al.]. Effect of Baoshenfang Formula on Podocyte Injury via Inhibiting the NOX-4ROSp38 Pathway in Diabetic Nephropathy. Journal of Diabetes Research. 2019. Vol. 2019, no. 2019, pp.1-16.
https://search.emarefa.net/detail/BIM-1172867
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172867