Transcriptome Analysis Reveals Dynamic Fat Accumulation in the Walnut Kernel
Joint Authors
Zhao, Shuang
Zhang, Xuemei
Su, Yanping
Chen, Yilan
Liu, Yang
Sun, Meng
Qi, Guohui
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-28
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Walnut (Juglans regia L.) is an important woody oilseed species cultivated throughout the world.
In this study, comparative transcript profiling was performed using high-throughput RNA sequencing technology at the following three stages of walnut fat synthesis in the “Lvling” walnut cultivar: the initial developmental stage (L1), the fast developing stage (L2), and the last developing stage (L3).
A total of 68.18 GB of data were obtained on the three developmental stages, and 92% to 94% of clean data were able to be located to the reference genome.
Further comparisons of the transcripts in the three libraries revealed that 724, 2027, and 4817 genes were differentially expressed between the L2 and L1 (L2vsL1), L3 and L2 (L3vsL2), and L3 and L1 (L3vsL1) samples, respectively.
Through the GO gene enrichment analysis, differentially expressed genes (DEGs) in L2vsL1, L3vsL2, and L3vsL1 were enriched into 3, 0, and 2 functional categories, respectively.
According to the KEGG enrichment analysis, DEGs in L2vsL1, L3vsL2, and L3vsL1 were annotated into 77, 110, and 3717 taxonomic metabolic pathways in the KEGG database, respectively.
Next, we analyzed expression levels of genes related to fat synthesis.
Our results indicated that ACCase, LACS, and FAD7 were the key genes related to fat synthesis.
The high-throughput transcriptome sequencing of walnut in different developmental stages has greatly enriched the current genomic available resources.
The comparison of DEGs under different developmental stages identified a wealth of candidate genes involved in fat synthesis, which will facilitate further genetic improvement and molecular studies of the walnut.
American Psychological Association (APA)
Zhao, Shuang& Zhang, Xuemei& Su, Yanping& Chen, Yilan& Liu, Yang& Sun, Meng…[et al.]. 2018. Transcriptome Analysis Reveals Dynamic Fat Accumulation in the Walnut Kernel. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1172898
Modern Language Association (MLA)
Zhao, Shuang…[et al.]. Transcriptome Analysis Reveals Dynamic Fat Accumulation in the Walnut Kernel. International Journal of Genomics No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1172898
American Medical Association (AMA)
Zhao, Shuang& Zhang, Xuemei& Su, Yanping& Chen, Yilan& Liu, Yang& Sun, Meng…[et al.]. Transcriptome Analysis Reveals Dynamic Fat Accumulation in the Walnut Kernel. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1172898
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172898