Multiple Regression Analysis Reveals MicroRNA Regulatory Networks in Oryza sativa under Drought Stress
Joint Authors
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-10-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Drought is a major abiotic stress that reduces rice development and yield.
miRNAs (microRNAs) are known to mediate posttranscriptional regulation under drought stress.
Although the importance of individual miRNAs has been established, the crosstalks between miRNAs and mRNAs remain unearthed.
Here we performed microarray analysis of miRNAs and matched mRNA expression profiles of drought-treated rice cultivar Nipponbare.
Drought-responsive miRNA-mRNA regulations were identified by a combination of a partial least square (PLS) regression approach and sequence-based target prediction.
A drought-induced network with 13 miRNAs and 58 target mRNAs was constructed, and four miRNA coregulatory modules were revealed.
Functional analysis suggested that drought-response miRNA targets are enriched in hormone signaling, lipid and carbohydrate metabolism, and antioxidant defense.
13 candidate miRNAs and target genes were validated by RT-qPCR, hierarchical clustering, and ROC analysis.
Two target genes (DWARF-3 and P0651G05.2) of miRNA coregulatory modules were further verified by RLM-5′ RACE.
Together, our integrative study of miRNA-mRNA interaction provided attractive candidates that will help elucidate the drought-response mechanisms in Oryza sativa.
American Psychological Association (APA)
Chen, Jiajia& Li, Liangzhi. 2018. Multiple Regression Analysis Reveals MicroRNA Regulatory Networks in Oryza sativa under Drought Stress. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1172922
Modern Language Association (MLA)
Chen, Jiajia& Li, Liangzhi. Multiple Regression Analysis Reveals MicroRNA Regulatory Networks in Oryza sativa under Drought Stress. International Journal of Genomics No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1172922
American Medical Association (AMA)
Chen, Jiajia& Li, Liangzhi. Multiple Regression Analysis Reveals MicroRNA Regulatory Networks in Oryza sativa under Drought Stress. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1172922
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172922