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Advanced Glycation End-Products and Hyperglycemia Increase Angiopoietin-2 Production by Impairing Angiopoietin-1-Tie-2 System
Joint Authors
Cordera, Renzo
Nicolò, Massimo
Maggi, Davide
Traverso, Carlo E.
Puddu, Alessandra
Sanguineti, Roberta
Viviani, Giorgio Luciano
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The angiopoietin-Tie-2 system plays a crucial role in the maintenance of endothelial integrity.
Hyperglycemia and advanced glycation end-products (AGEs) are involved in endothelial cell dysfunction responsible of the pathogenesis of microvascular complications of diabetes.
Here, we investigated whether glycated serum (GS) or hyperglycemia (HG) affect the angiopoietin-Tie-2 system in the microvascular endothelial cells HMEC-1.
We found that culture for 5 days in the presence of AGEs and HG (alone or in combination) decreased cell proliferation, increased reactive oxygen species (ROS) production, and reduced ratio between the oxidized and the reduced form of glutathione.
Since angiopoietin-1 (Ang-1) signaling regulates angiopoietin-2 (Ang-2) expression through inactivation of the forkhead transcription factor FoxO1, we investigated intracellular signaling of Ang-1 and expression of Ang-2.
HG and AGEs reduced phosphorylation of Akt and abrogated phosphorylation of FoxO1 induced by Ang-1 without affecting neither Tie-2 expression nor its activation.
Furthermore, AGEs and/or HG induced nuclear translocation of FoxO1 and increased Ang-2 production.
In conclusion, we demonstrated that both hyperglycemia and AGEs affect the angiopoietin-Tie-2 system by impairing Ang-1/Tie-2 signaling and by increasing Ang-2 expression.
These results suggest that therapeutic strategies useful in preventing or delaying the onset of diabetic vascular complications should be aimed to preserve Ang-1 signaling.
American Psychological Association (APA)
Puddu, Alessandra& Sanguineti, Roberta& Maggi, Davide& Nicolò, Massimo& Traverso, Carlo E.& Cordera, Renzo…[et al.]. 2019. Advanced Glycation End-Products and Hyperglycemia Increase Angiopoietin-2 Production by Impairing Angiopoietin-1-Tie-2 System. Journal of Diabetes Research،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1173096
Modern Language Association (MLA)
Puddu, Alessandra…[et al.]. Advanced Glycation End-Products and Hyperglycemia Increase Angiopoietin-2 Production by Impairing Angiopoietin-1-Tie-2 System. Journal of Diabetes Research No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1173096
American Medical Association (AMA)
Puddu, Alessandra& Sanguineti, Roberta& Maggi, Davide& Nicolò, Massimo& Traverso, Carlo E.& Cordera, Renzo…[et al.]. Advanced Glycation End-Products and Hyperglycemia Increase Angiopoietin-2 Production by Impairing Angiopoietin-1-Tie-2 System. Journal of Diabetes Research. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1173096
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173096