Molecular Characterization of Newcastle Disease Virus from Backyard Poultry Farms and Live Bird Markets in Kenya
Joint Authors
Lichoti, Jacqueline Kasiiti
Agwanda, Bernard
Ommeh, Sheila Cecily
Ogali, Irene N.
Wamuyu, Lucy W.
Mungube, Erick O.
Source
International Journal of Microbiology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-08-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Newcastle disease (ND) is a serious disease of poultry that causes significant economic losses.
Despite rampant ND outbreaks that occur annually in Kenya, the information about the NDV circulating in Kenya is still scarce.
We report the first countrywide study of NDV in Kenya.
Our study is aimed at evaluating the genetic characteristics of Newcastle disease viruses obtained from backyard poultry in farms and live bird markets in different regions of Kenya.
We sequenced and analyzed fusion (F) protein gene, including the cleavage site, of the obtained viruses.
We aligned and compared study sequences with representative NDV of different genotypes from GenBank.
The fusion protein cleavage site of all the study sequences had the motif 112RRQKRFV118 indicating their velogenic nature.
Phylogenetic analysis revealed that the NDV from various sites in Kenya was highly similar genetically and that it clustered together with NDV of genotype V.
The study samples were 96% similar to previous Ugandan and Kenyan viruses grouped in subgenotype Vd This study points to possible circulation of NDV of similar genetic characteristics between backyard poultry farms and live bird markets in Kenya.
The study also suggests the possible spread of velogenic NDV between Kenya and Uganda possibly through cross-border live bird trade.
Our study provides baseline information on the genetic characteristics of NDV circulating in the Kenyan poultry population.
This highlights the need for the ND control programmes to place more stringent measures on cross-border trade of live bird markets and poultry products to prevent the introduction of new strains of NDV that would otherwise be more difficult to control.
American Psychological Association (APA)
Ogali, Irene N.& Wamuyu, Lucy W.& Lichoti, Jacqueline Kasiiti& Mungube, Erick O.& Agwanda, Bernard& Ommeh, Sheila Cecily. 2018. Molecular Characterization of Newcastle Disease Virus from Backyard Poultry Farms and Live Bird Markets in Kenya. International Journal of Microbiology،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1173299
Modern Language Association (MLA)
Ogali, Irene N.…[et al.]. Molecular Characterization of Newcastle Disease Virus from Backyard Poultry Farms and Live Bird Markets in Kenya. International Journal of Microbiology No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1173299
American Medical Association (AMA)
Ogali, Irene N.& Wamuyu, Lucy W.& Lichoti, Jacqueline Kasiiti& Mungube, Erick O.& Agwanda, Bernard& Ommeh, Sheila Cecily. Molecular Characterization of Newcastle Disease Virus from Backyard Poultry Farms and Live Bird Markets in Kenya. International Journal of Microbiology. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1173299
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173299