Effect of Mn3O4 Nanoparticles on Lipopolysaccharide-Induced Inflammatory Factors in the Human Tendon Cells and Its Mechanism
Joint Authors
Fu, Shangjun
He, Zongyun
Tang, Yongfeng
Lan, Bo
Source
International Journal of Polymer Science
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
To investigate the effect of Mn3O4 nanoparticles (Mn3O4NPs) on inflammatory factors induced by lipopolysaccharide (LPS) in human tendon cells and its mechanism.
Methods.
The Mn3O4NPs were synthesized by a hydrothermal method.
RT-qPCR was used to detect the expression levels of miRNAs related to inflammation in human tendon cells.
The expression level of NLRP1 (NOD-like receptor containing pyrin domain 1) was measured by Western blotting.
ELISA assay was used to measure the level of TNF-α, IL-1β, IL-4, and IL-10.
The relationship between miR-181a-5p and NLRP1 was verified by dual-luciferase reporter assay.
Results.
Mn3O4NPs produced in this study were brown spherical particles with an average size of 7-10 nm.
Mn3O4NP treatment significantly reduced the levels of TNF-α and IL-1β but increased the levels of IL-4 and IL-10 in the human tendon cells induced by LPS.
In addition, Mn3O4NP treatment remarkably increased the expression level of miR-181a-5p.
NLRP1 is one of the targets of miR-181a-5p, and miR-181a-5p downregulated its expression.
Further study showed that Mn3O4NPs could alleviate the inflammatory response of human tendon cells induced by LPS by upregulating miR-181a-5p and thus downregulating the expression of NLRP1.
Conclusion.
Mn3O4NPs affect the expression of inflammatory cytokines in the human tendon cells induced by LPS by modulating the molecular axis of miR-181a-5p/NLRP1.
American Psychological Association (APA)
Fu, Shangjun& He, Zongyun& Tang, Yongfeng& Lan, Bo. 2020. Effect of Mn3O4 Nanoparticles on Lipopolysaccharide-Induced Inflammatory Factors in the Human Tendon Cells and Its Mechanism. International Journal of Polymer Science،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1173725
Modern Language Association (MLA)
Fu, Shangjun…[et al.]. Effect of Mn3O4 Nanoparticles on Lipopolysaccharide-Induced Inflammatory Factors in the Human Tendon Cells and Its Mechanism. International Journal of Polymer Science No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1173725
American Medical Association (AMA)
Fu, Shangjun& He, Zongyun& Tang, Yongfeng& Lan, Bo. Effect of Mn3O4 Nanoparticles on Lipopolysaccharide-Induced Inflammatory Factors in the Human Tendon Cells and Its Mechanism. International Journal of Polymer Science. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1173725
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173725