Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound
Joint Authors
Wang, Qi
Zhu, Guanya
Cao, Xiaozan
Dong, Jiaoyun
Song, Fei
Niu, Yiwen
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-08
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Advanced glycosylation end products (AGEs) accumulate in diabetic wounds.
Interactions between AGEs and their receptor (RAGE) leads to dermatologic problems in diabetes.
Macrophage, which plays important roles in wound healing, highly expresses RAGE.
Therefore, we investigated whether RAGE-expressing macrophages might be responsible for impaired wound healing on diabetes.
We used anti-RAGE antibody applied topically on diabetic wounds.
After confirming that wound healing was improved in anti-RAGE antibody group compared with normal mice, our results showed that macrophages appeared insufficient in the early stage and fading away slowly in the later proliferative phase compared with the control group, which was ameliorated in anti-RAGE antibody-applied wounds.
Blocking AGE-RAGE signaling also increased neutrophils phagocytized by macrophages and promoted the phenotypic switch of macrophages from proinflammatory to prohealing activities.
In vitro, phagocytosis of THP-1 (M0) and lipopolysaccharide- (LPS-) induced (M1) macrophages was impaired by treatment with AGEs, while IL-4- and IL-13-induced (M2) macrophages was not.
Finally, AGEs increased the proinflammatory response of M1 macrophages, while inhibiting the polarization and anti-inflammatory functions of M2 macrophages.
In conclusion, inhibition of AGE-RAGE signaling improved functional disorders of macrophages in the early inflammatory phase, which promoted the healing of wounds in diabetic mice.
American Psychological Association (APA)
Wang, Qi& Zhu, Guanya& Cao, Xiaozan& Dong, Jiaoyun& Song, Fei& Niu, Yiwen. 2017. Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound. Journal of Diabetes Research،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1173767
Modern Language Association (MLA)
Wang, Qi…[et al.]. Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound. Journal of Diabetes Research No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1173767
American Medical Association (AMA)
Wang, Qi& Zhu, Guanya& Cao, Xiaozan& Dong, Jiaoyun& Song, Fei& Niu, Yiwen. Blocking AGE-RAGE Signaling Improved Functional Disorders of Macrophages in Diabetic Wound. Journal of Diabetes Research. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1173767
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173767