Acute Elevated Glucose Promotes Abnormal Action Potential-Induced Ca2+ Transients in Cultured Skeletal Muscle Fibers
Joint Authors
Banks, Quinton
Schneider, Martin F.
Hernández-Ochoa, Erick O.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-08-01
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A common comorbidity of diabetes is skeletal muscle dysfunction, which leads to compromised physical function.
Previous studies of diabetes in skeletal muscle have shown alterations in excitation-contraction coupling (ECC)—the sequential link between action potentials (AP), intracellular Ca2+ release, and the contractile machinery.
Yet, little is known about the impact of acute elevated glucose on the temporal properties of AP-induced Ca2+ transients and ionic underlying mechanisms that lead to muscle dysfunction.
Here, we used high-speed confocal Ca2+ imaging to investigate the temporal properties of AP-induced Ca2+ transients, an intermediate step of ECC, using an acute in cellulo model of uncontrolled hyperglycemia (25 mM, 48 h.).
Control and elevated glucose-exposed muscle fibers cultured for five days displayed four distinct patterns of AP-induced Ca2+ transients (phasic, biphasic, phasic-delayed, and phasic-slow decay); most control muscle fibers show phasic AP-induced Ca2+ transients, while most fibers exposed to elevated D-glucose displayed biphasic Ca2+ transients upon single field stimulation.
We hypothesize that these changes in the temporal profile of the AP-induced Ca2+ transients are due to changes in the intrinsic excitable properties of the muscle fibers.
We propose that these changes accompany early stages of diabetic myopathy.
American Psychological Association (APA)
Hernández-Ochoa, Erick O.& Banks, Quinton& Schneider, Martin F.. 2017. Acute Elevated Glucose Promotes Abnormal Action Potential-Induced Ca2+ Transients in Cultured Skeletal Muscle Fibers. Journal of Diabetes Research،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1173816
Modern Language Association (MLA)
Hernández-Ochoa, Erick O.…[et al.]. Acute Elevated Glucose Promotes Abnormal Action Potential-Induced Ca2+ Transients in Cultured Skeletal Muscle Fibers. Journal of Diabetes Research No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1173816
American Medical Association (AMA)
Hernández-Ochoa, Erick O.& Banks, Quinton& Schneider, Martin F.. Acute Elevated Glucose Promotes Abnormal Action Potential-Induced Ca2+ Transients in Cultured Skeletal Muscle Fibers. Journal of Diabetes Research. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1173816
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173816