Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T
Joint Authors
Zhang, Junrong
Liu, Rui
Liu, Hongxiang
Xu, Baofeng
Huang, Sijun
Zhu, Jinming
Guo, Yi
Xu, Li
Source
International Journal of Polymer Science
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Acute myocardial infarction (AMI) causes irreversible injury to cardiomyocytes in a short time and may result in various complications, severely threatening patient safety.
Therefore, it is necessary to predict the possibility of AMI in the prophase.
Prognostic detection of biomarkers that specifically reflect myocardial damage in a patient’s blood has become an essential mediating measure to prevent the serious occurrence of AMI.
The present study is aimed at exploring a novel sensing system with high specificity and precision based on magnetic microspheres developed to detect cardiac troponin T (cTnT), which is the most specific diagnostic marker for AMI in cardiovascular diseases.
Naive human cTnT protein in serum samples and antigens on functional magnetic microspheres will competitively bind with limited specific antibodies.
After rapid removal of heterogeneous elements in the sera using a magnetic separator, fluorescein isothiocyanate-labeled immunoglobulin G is added to react with specific antibodies on the magnetic microspheres.
Then, a flow cytometer is used to collect signals of different fluorescence intensities.
The results show that the method is characterized by economy, high accuracy, and novelty.
It can be used for the detection of cTnT in blood at 1.7–106.1 ng/mL, with a detection limit of 0.5 ng/mL.
Thus, the proposed sensor improves the accuracy and efficiency of diagnosis before clinical deterioration of AMI.
American Psychological Association (APA)
Zhang, Junrong& Liu, Hongxiang& Xu, Baofeng& Huang, Sijun& Liu, Rui& Zhu, Jinming…[et al.]. 2020. Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T. International Journal of Polymer Science،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1173898
Modern Language Association (MLA)
Zhang, Junrong…[et al.]. Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T. International Journal of Polymer Science No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1173898
American Medical Association (AMA)
Zhang, Junrong& Liu, Hongxiang& Xu, Baofeng& Huang, Sijun& Liu, Rui& Zhu, Jinming…[et al.]. Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T. International Journal of Polymer Science. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1173898
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173898