Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL6 Mice
Joint Authors
Long, Yang
Chen, Tao
Tian, Hao-Ming
Jia, Dan
Wei, Libin
Yang, Yumei
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
In the current study, we developed a liver-specific GGT-overexpressing mice model by rapid injection pLIVE-GGT vector through tail vein and investigated the effects of GGT elevation on glucose metabolism and insulin sensitivity.
The serum GGT activity was significantly increased after 7 days of pLIVE-GGT1 vector delivery and lasted for about 3 weeks.
GGT overexpression reduced the levels of GSSG and GSH in the liver and serum and had no effects on total antioxidative capacity in the liver, kidney, and skeletal muscle except for the pancreas.
Increased GGT activity had no effect on the glucose tolerance but could facilitate blood glucose lowering after intraperitoneal insulin administration.
The results of Western blotting showed that increased GGT activity enhanced insulin-induced AKT phosphorylation at Ser473.
Furthermore, GGT inhibitor could attenuate the changes of insulin-induced blood glucose uptake and AKT phosphorylation in the liver.
In summary, the present study developed a liver-specific GGT-overexpressing mice model and found that GGT elevation in short term had no effects on glucose metabolism but could increase insulin sensitivity through enhancing the activity of insulin signaling pathway.
American Psychological Association (APA)
Long, Yang& Jia, Dan& Wei, Libin& Yang, Yumei& Tian, Hao-Ming& Chen, Tao. 2017. Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL6 Mice. Journal of Diabetes Research،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1173949
Modern Language Association (MLA)
Long, Yang…[et al.]. Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL6 Mice. Journal of Diabetes Research No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1173949
American Medical Association (AMA)
Long, Yang& Jia, Dan& Wei, Libin& Yang, Yumei& Tian, Hao-Ming& Chen, Tao. Liver-Specific Overexpression of Gamma-Glutamyltransferase Ameliorates Insulin Sensitivity of Male C57BL6 Mice. Journal of Diabetes Research. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1173949
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173949