Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy
Joint Authors
Su, Guanfang
Gong, Qiaoyun
Xie, Jia’nan
Liu, Yang
Li, Ying
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The pathological mechanisms of diabetic retinopathy (DR), a leading cause of blindness in adults with diabetes mellitus, remain incompletely understood.
Because microRNAs (miRNAs) represent effective DR therapeutic targets, we identified aberrantly expressed miRNAs associated with cellular dysfunction in early DR and detected their potential targets.
We exposed human retinal endothelial cells (HRECs) and a cell line of retinal pigment epithelial (RPE) cells to high glucose (25 mmol/L, 1–7 days) to mimic DR progression and used streptozotocin-injected rats (4–8 weeks) for an in vivo diabetes model.
HREC/RPE viability decreased after 24 h incubation and diminished further over 6 days, and Hoechst staining revealed hyperglycemia-induced HREC/RPE apoptosis.
Although miR-124/-125b expression decreased with DR progression in vitro and in vivo, miR-135b/-199a levels decreased in retinal cells under hyperglycemia exposure, but increased in diabetic retinas.
Moreover, miR-145/-146a expression decreased gradually in high-glucose-treated HRECs, but increased in hyperglycemia-exposed RPE cells and in diabetic rats.
Our findings suggested that aberrant miRNA expression could be involved in hyperglycemia-induced retinal-cell dysfunction, and the identified miRNAs might vary in different retinal layers, with expression changes associated with DR development.
Therefore, miRNA modulation and the targeting of miRNA effects on transcription factors could represent novel and effective DR-treatment strategies.
American Psychological Association (APA)
Gong, Qiaoyun& Xie, Jia’nan& Liu, Yang& Li, Ying& Su, Guanfang. 2017. Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy. Journal of Diabetes Research،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1174326
Modern Language Association (MLA)
Gong, Qiaoyun…[et al.]. Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy. Journal of Diabetes Research No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1174326
American Medical Association (AMA)
Gong, Qiaoyun& Xie, Jia’nan& Liu, Yang& Li, Ying& Su, Guanfang. Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy. Journal of Diabetes Research. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1174326
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174326