Extended-Spectrum β-Lactamase (ESBL) Genotypes among Multidrug-Resistant Uropathogenic Escherichia coli Clinical Isolates from a Teaching Hospital of Nepal
Joint Authors
Parajuli, Narayan Prasad
Joshi, Govardhan
Rijal, Basista
Pandit, Roshan
Shrestha, Sumesh Shreekhanda
Awal, Balkrishna
Source
Interdisciplinary Perspectives on Infectious Diseases
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Urinary tract infections (UTI) represent the most common bacterial infections among patients visiting outpatient clinics of healthcare centers in Nepal.
However, treatment of such infections is compounded by emergence and spread of multidrug-resistant uropathogens associated with extended-spectrum β-lactamases (ESBLs).
In this study, we aimed to investigate the burden of antimicrobial resistance and occurrence of ESBL genes among clinical isolates of uropathogenic Escherichia coli at a tertiary care teaching hospital of Nepal.
During the study period, we processed a total of 1,626 urinary tract specimens, isolated significant bacterial pathogens, and investigated their antimicrobial susceptibilities.
Escherichia coli (n = 154), the predominant pathogen associated with UTI, was further investigated for the existence of ESBL enzymes by using conventional phenotypic as well as molecular approaches.
Among suspected cases of UTI, we found that 15.2% were having UTI and female patients of the reproductive age group were more affected (p<0.05).
Escherichia coli (154, 62.1%) was the key uropathogen, and majority (∼64.9%) of them were multidrug resistant (MDR).
Among MDR E.
coli isolates, 40.3% were producing extended-spectrum β-lactamases (ESBLs).
bla-TEM (83.8%), bla-CTX-M (66.1%), and bla-SHV (4.8%) were common ESBL genotypes.
Nitrofurantoin, gentamycin, and imipenem were the most effective antibiotics for ESBL-producing Escherichia coli isolates.
It indicates that the high rates of multidrug-resistant Escherichia coli are frequent causes of UTI in our hospital.
Nitrofurantoin and aminoglycosides are the most useful first-line drugs to be used in the cases of UTI.
We recommend the regular investigation of drug resistance among all isolates and develop a useful antibiotic prescription policy in our country.
American Psychological Association (APA)
Pandit, Roshan& Awal, Balkrishna& Shrestha, Sumesh Shreekhanda& Joshi, Govardhan& Rijal, Basista& Parajuli, Narayan Prasad. 2020. Extended-Spectrum β-Lactamase (ESBL) Genotypes among Multidrug-Resistant Uropathogenic Escherichia coli Clinical Isolates from a Teaching Hospital of Nepal. Interdisciplinary Perspectives on Infectious Diseases،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1174346
Modern Language Association (MLA)
Pandit, Roshan…[et al.]. Extended-Spectrum β-Lactamase (ESBL) Genotypes among Multidrug-Resistant Uropathogenic Escherichia coli Clinical Isolates from a Teaching Hospital of Nepal. Interdisciplinary Perspectives on Infectious Diseases No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1174346
American Medical Association (AMA)
Pandit, Roshan& Awal, Balkrishna& Shrestha, Sumesh Shreekhanda& Joshi, Govardhan& Rijal, Basista& Parajuli, Narayan Prasad. Extended-Spectrum β-Lactamase (ESBL) Genotypes among Multidrug-Resistant Uropathogenic Escherichia coli Clinical Isolates from a Teaching Hospital of Nepal. Interdisciplinary Perspectives on Infectious Diseases. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1174346
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174346