Aldose Reductase Inhibitor Protects against Hyperglycemic Stress by Activating Nrf2-Dependent Antioxidant Proteins
Joint Authors
Shukla, Kirtikar
Pal, Pabitra Bikash
Sonowal, Himangshu
Srivastava, Satish K.
Ramana, Kota V.
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-06-27
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We have shown earlier that pretreatment of cultured cells with aldose reductase (AR) inhibitors prevents hyperglycemia-induced mitogenic and proinflammatory responses.
However, the effects of AR inhibitors on Nrf2-mediated anti-inflammatory responses have not been elucidated yet.
We have investigated how AR inhibitor fidarestat protects high glucose- (HG-) induced cell viability changes by increasing the expression of Nrf2 and its dependent phase II antioxidant enzymes.
Fidarestat pretreatment prevents HG (25 mM)-induced Thp1 monocyte viability.
Further, treatment of Thp1 monocytes with fidarestat caused a time-dependent increase in the expression as well as the DNA-binding activity of Nrf2.
In addition, fidarestat augmented the HG-induced Nrf2 expression and activity and also upregulated the expression of Nrf2-dependent proteins such as hemeoxygenase-1 (HO1) and NQO1 in Thp1 cells.
Similarly, treatment with AR inhibitor also induced the expression of Nrf2 and HO1 in STZ-induced diabetic mice heart and kidney tissues.
Further, AR inhibition increased the HG-induced expression of antioxidant enzymes such as SOD and catalase and activation of AMPK-α1 in Thp1 cells.
Our results thus suggest that pretreatment with AR inhibitor prepares the monocytes against hyperglycemic stress by overexpressing the Nrf2-dependent antioxidative proteins.
American Psychological Association (APA)
Shukla, Kirtikar& Pal, Pabitra Bikash& Sonowal, Himangshu& Srivastava, Satish K.& Ramana, Kota V.. 2017. Aldose Reductase Inhibitor Protects against Hyperglycemic Stress by Activating Nrf2-Dependent Antioxidant Proteins. Journal of Diabetes Research،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1174628
Modern Language Association (MLA)
Shukla, Kirtikar…[et al.]. Aldose Reductase Inhibitor Protects against Hyperglycemic Stress by Activating Nrf2-Dependent Antioxidant Proteins. Journal of Diabetes Research No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1174628
American Medical Association (AMA)
Shukla, Kirtikar& Pal, Pabitra Bikash& Sonowal, Himangshu& Srivastava, Satish K.& Ramana, Kota V.. Aldose Reductase Inhibitor Protects against Hyperglycemic Stress by Activating Nrf2-Dependent Antioxidant Proteins. Journal of Diabetes Research. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1174628
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174628