Bioguided Isolation and Structure Identification of Acetylcholinesterase Enzyme Inhibitors from Drynariae Rhizome
Joint Authors
Liu, Ming-Yang
Zeng, Fan
Shen, Yue
Wang, Yu-Ying
Zhang, Ning
Geng, Fang
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Drynariae Rhizome, widely distributed in southern China, was clinically used as a traditional treatment for cognitive disfunction, such as Alzheimer’s disease (AD).
The aim of our work was to evaluate the AChE inhibition activities of extracts of Drynariae Rhizome and pure compounds using a bioguided fractionation procedure.
The classical approach for screening potential AChE inhibitors was developed by Ellman.
However, the background color of compounds or herb extracts remained uncertain and frequently interfered with the detection of the secondary reaction, thereby easily yielding false positive or false negative results.
Here, a high-throughput assay monitoring the transformation of iodized choline from iodized acetylcholine catalyzed by AChE was established based on UPLC-MS/MS.
The bioguided fractionation of the extract using this method resulted in the isolation of eight AChE inhibitory flavonoids, including naringenin, eriodictyol, kaempferol, luteolin, astragalin, luteolin-7-O-β-D-glucoside, naringin, and neoeriocitrin, with the IC50 values of 3.81 ± 0.21 μM, 7.19 ± 0.62 μM, 11.09 ± 1.02 μM, 17.26 ± 0.23 μM, 18.24 ± 2.33 μM, 17.13 ± 1.02 μM, 26.4 ± 1.17 μM, and 22.49 ± 1.25 μM.
It is assumed that the identified flavonoids contribute to the AChE inhibition activity of Drynariae Rhizome.
These results are in agreement with the traditional uses of Drynariae Rhizome for AD.
American Psychological Association (APA)
Liu, Ming-Yang& Zeng, Fan& Shen, Yue& Wang, Yu-Ying& Zhang, Ning& Geng, Fang. 2020. Bioguided Isolation and Structure Identification of Acetylcholinesterase Enzyme Inhibitors from Drynariae Rhizome. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1174703
Modern Language Association (MLA)
Liu, Ming-Yang…[et al.]. Bioguided Isolation and Structure Identification of Acetylcholinesterase Enzyme Inhibitors from Drynariae Rhizome. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1174703
American Medical Association (AMA)
Liu, Ming-Yang& Zeng, Fan& Shen, Yue& Wang, Yu-Ying& Zhang, Ning& Geng, Fang. Bioguided Isolation and Structure Identification of Acetylcholinesterase Enzyme Inhibitors from Drynariae Rhizome. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1174703
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174703