Quantification of Alfacalcidol Tablets Dissolution Content by Chemical Derivatization and LC-MS
Joint Authors
Liu, Yang
He, Lan
Chen, Xi
Yuan, Song
Liu, Wanhui
Zhang, Qingsheng
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-07
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Application of liquid chromatography-mass spectrometry (LC-MS) in analyzing the content of alfacalcidol tablets dissolution faces big challenges due to the low amount of alfacalcidol in each tablet and the low ionization efficacy of the compound with electrospray ionization (ESI) or atmospheric-pressure chemical ionization (APCI).
Here, extraction, derivatization, and LC-MS quantitation method have been developed and validated for measuring alfacalcidol tablets dissolution content.
After alfacalcidol dissolution solution was extracted with dichloromethane to remove surfactant and inorganic salts, alfacalcidol was then derivatized via a Cookson reagent, 4-phenyl-1, 2, 4-triazoline-3, 5-dione (PTAD), under ambient conditions.
Alfacalcidol derivative was successfully analyzed by LC-MS.
Limit of detection (LOD) of the derivatized alfacalcidol was improved 100 times (0.01 μg/mL) compared with the nontreated compound (1 μg/mL).
The new method was then validated following International Conference on Harmonization (ICH) guidance.
The method shows a good linearity with r2 > 0.99.
Interday and intraday reproducibility was 3.3% and 7.9%, respectively.
This procedure can be used in quantification of alfacalcidol tablets dissolution content and corresponding pharmaceutical quality control.
American Psychological Association (APA)
Liu, Yang& Chen, Xi& Yuan, Song& Liu, Wanhui& He, Lan& Zhang, Qingsheng. 2020. Quantification of Alfacalcidol Tablets Dissolution Content by Chemical Derivatization and LC-MS. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1174781
Modern Language Association (MLA)
Liu, Yang…[et al.]. Quantification of Alfacalcidol Tablets Dissolution Content by Chemical Derivatization and LC-MS. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1174781
American Medical Association (AMA)
Liu, Yang& Chen, Xi& Yuan, Song& Liu, Wanhui& He, Lan& Zhang, Qingsheng. Quantification of Alfacalcidol Tablets Dissolution Content by Chemical Derivatization and LC-MS. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1174781
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174781