A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics
Joint Authors
Xu, Tong
Li, Xiaoqi
Huang, Mengmeng
Wang, Qi
Li, Chao
Tian, Gang
Chen, Yan
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A preferable approach of a combination of a multiwavelength fusion HPLC fingerprint and chemometrics for the quality control of Xiaoer Chiqiao Qingre granules (XCQG) was established in this study.
A single-wavelength HPLC fingerprint was performed to identify 18 peaks as common peaks in the beginning, and 12 of them were recognized by HPLC-Q/TOF-MS.
To overcome the limitation of the single-wavelength HPLC fingerprint, a three-wavelength (230 nm, 250 nm, and 330 nm) fusion fingerprint was established for a more thorough quality assessment.
Six main active ingredients (geniposide, paeoniflorin, forsythin, forsythoside A, baicalin, and wogonoside) were selected as chemical markers for simultaneous quantitative analysis, while the results indicated that the content of other five ingredients except forsythoside A presented comparatively stable.
Chemometrics including hierarchical cluster analysis (HCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were performed to evaluate the homogeneity and heterogeneity of sixteen batches of XCQG.
The results of the multiwavelength fingerprint were clearly classified into two clusters by HCA, whereas the single-wavelength fingerprint showed no distinct difference between them.
OPLS-DA was further employed to prove that the above six main active ingredients made great contributions to clustering.
In summary, this integrated analysis provided a better promoted and more comprehensive method to control the quality of XCQG.
American Psychological Association (APA)
Xu, Tong& Li, Xiaoqi& Huang, Mengmeng& Wang, Qi& Li, Chao& Tian, Gang…[et al.]. 2020. A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1174819
Modern Language Association (MLA)
Xu, Tong…[et al.]. A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1174819
American Medical Association (AMA)
Xu, Tong& Li, Xiaoqi& Huang, Mengmeng& Wang, Qi& Li, Chao& Tian, Gang…[et al.]. A Preferable Approach for the Quality Control of Xiaoer Chiqiao Qingre Granules Based on the Combination of Chromatographic Fingerprints and Chemometrics. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1174819
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174819