Simultaneous Determination of Phenolic Acids, Anthraquinones, Flavonoids, and Triterpenes of Cynomorii Herba in Different Harvest Times by LC-MSMS
Joint Authors
Chang, Yanxu
Li, Yuhong
Jin, Hua
Ma, Lin
Tang, Ge
Li, Jin
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
A sensitive and accurate method was developed for the simultaneous determination of twelve components including phenolic acids (gallic acid, protocatechuic aldehyde, protocatechuic acid, and ferulic acid), flavonoids (catechin, epicatechin, rutin, luteolin, luteolin-7-glucoside, and epicatechin gallate), anthraquinones (emodin), and triterpenes (ursolic acid) in Cynomorii herba in different harvest times by liquid chromatography tandem mass spectrometry (LC-MS/MS).
The chromatographic separation was achieved on an Eclipse plus C18 (3.0 mm × 50 mm, 1.8 μm) column at 40°C.
The mobile phase consisted of acetonitrile and 0.05% formic acid with a gradient elution at a flow rate of 0.4 mL·min −1.
Under the optimized conditions, there was good linear relation (r ≥ 0.9944) and satisfactory precision (RSD values less than 5.65%).
The recoveries of the twelve components were in the range of 93.5–105%.
Moreover, the limits of detection (LOD) ranged from 0.003 to 21 ng mL−1 for the twelve analytes.
In conclusion, the validated method was successfully applied to analyze the change regularity of the twelve components of Cynomorii herba in different harvest times.
It provides a theoretical basis for choosing the suitable harvesting time of Cynomorii herba.
American Psychological Association (APA)
Jin, Hua& Tang, Ge& Li, Jin& Ma, Lin& Li, Yuhong& Chang, Yanxu. 2020. Simultaneous Determination of Phenolic Acids, Anthraquinones, Flavonoids, and Triterpenes of Cynomorii Herba in Different Harvest Times by LC-MSMS. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1174971
Modern Language Association (MLA)
Jin, Hua…[et al.]. Simultaneous Determination of Phenolic Acids, Anthraquinones, Flavonoids, and Triterpenes of Cynomorii Herba in Different Harvest Times by LC-MSMS. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1174971
American Medical Association (AMA)
Jin, Hua& Tang, Ge& Li, Jin& Ma, Lin& Li, Yuhong& Chang, Yanxu. Simultaneous Determination of Phenolic Acids, Anthraquinones, Flavonoids, and Triterpenes of Cynomorii Herba in Different Harvest Times by LC-MSMS. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1174971
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174971