Preparation of Keratin-Glycine Metal Complexes and Their Scavenging Activity for Superoxide Anion Radicals

Abstract EN

To address the problem of limited application of natural SOD, the development of SOD mimic enzymes is of great importance for bioantioxidation.

Herein, we report on a new type of biopolymer antioxidant with excellent scavenging activity for O2•-, keratin-glycine metal complexes (FK-GlyM, M = Zn, Cu, Mn, Ni).

They are prepared by feather keratin firstly combined with glycine and then metal ions.

Using FT-IR, TG, CD, and SEM, the performance of the obtained complexes (FK-GlyM) for scavenging O2•- is analysed and investigated.

Importantly, the scavenging activity of FK-GlyCu is excellent in all FK-GlyM, and FK-GlyCu60 has the most excellent anti-O2•- activity in all FK-GlyCux, of which EC50 and degree of simulation were, respectively, up to 4.5×10-3±0.0012 μmol/L and 911.1% compared with nature Cu, Zn-SOD.

Finally, its mechanism was also discussed.

In summary, this method about the simulation strategies will provide a novel idea for exploiting new-type biocompatible and highly reactive antioxidants.