LC-MSMS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation
Joint Authors
Tran, Cao-Son
Bui, Quang-Dong
Nguyen, Ngoc-Tho Thi
Dao, Minh-Hanh
Nguyen, Thach-Tung
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A rapid and effective method using QuEChERS-based sample preparation procedure and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated to determine progesterone in rabbit plasma.
The analyte was extracted from plasma by acetonitrile with phase partitioning by a mixture of magnesium sulfate and sodium chloride.
The supernatant was then directly injected into LC-MS/MS in a positive electrospray ionization mode and quantified using progesterone-d9 as the internal standard.
The method linearity was in the range from 1 ng/mL (LOQ) to 200 ng/mL.
Method recovery was from 86.0% to 103%, and repeatability was lower than 5.5%.
The plasma sample was stable for 12 weeks stored at 18 ± 2°C.
This method was applied to quantify progesterone in rabbit plasma in a pharmacokinetic study of two transdermal formulations: a reference drug and a eutectic-hydrogel system.
The data indicate that the eutectic-hydrogel system’s bioavailability was 1.5 times better than that of the reference drug, and the transdermal system is a potential drug delivery system for progesterone.
American Psychological Association (APA)
Tran, Cao-Son& Bui, Quang-Dong& Nguyen, Ngoc-Tho Thi& Dao, Minh-Hanh& Nguyen, Thach-Tung. 2020. LC-MSMS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1175010
Modern Language Association (MLA)
Tran, Cao-Son…[et al.]. LC-MSMS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1175010
American Medical Association (AMA)
Tran, Cao-Son& Bui, Quang-Dong& Nguyen, Ngoc-Tho Thi& Dao, Minh-Hanh& Nguyen, Thach-Tung. LC-MSMS Method for Rapid Quantification of Progesterone in Rabbit Plasma and Its Application in a Pharmacokinetic Study of the Transdermal Formulation. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1175010
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1175010