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A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”
Joint Authors
Yang, Yankun
Han, Shuang
Zhang, Junlan
Huang, Jianrong
Liu, Xiuxia
Bai, Zhonghu
Wang, Chunxin
Xue, Yifeng
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-12-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A chemiluminescent immunoassay for human serum osteocalcin, or bone Gla protein, was established using a double-antibody sandwich model.
Examination of the hook effect revealed that it was significantly reduced, and no hook effect was observed at an osteocalcin concentration of 4000 ng/mL.
The established method showed good analytical performance and thermal stability.
The limit of detection was 0.03 ng/mL.
The intra-assay coefficient of variation (CV) was 3.22%–5.64%, the interassay CV was 4.42%–7.25%, and the recovery rate was 93.22%–107.99%.
Cross-reactivity (CR) was not observed with bovine, rat, mouse, rabbit, or porcine samples.
The developed method showed a good correlation with the N-MID product from Roche.
In total, 1069 clinical patient samples were measured using the reagent kit developed in this study.
American Psychological Association (APA)
Han, Shuang& Xue, Yifeng& Zhang, Junlan& Huang, Jianrong& Liu, Xiuxia& Yang, Yankun…[et al.]. 2020. A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1175015
Modern Language Association (MLA)
Han, Shuang…[et al.]. A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1175015
American Medical Association (AMA)
Han, Shuang& Xue, Yifeng& Zhang, Junlan& Huang, Jianrong& Liu, Xiuxia& Yang, Yankun…[et al.]. A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1175015
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1175015