Pharmacokinetic and Lipidomic Assessment of the In Vivo Effects of Parishin A-Isorhynchophylline in Rat Migraine Models
Joint Authors
Ouyang, Hui
Yang, Shi-Lin
Ouyang, Hui
Zhou, Chaoqun
He, Mingzhen
Peng, Chunyan
Li, Zhifeng
Zhou, Maofu
Li, Yan
Feng, Yulin
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-07-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Migraine is a chronic brain disease that leads to periodic neurological attacks.
Parishin A and isorhynchophylline (PI) is the active monomer component extracted from the traditional antimigraine Chinese medicinal combination of Gastrodia and Uncaria, respectively.
In this study, using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) technology, we performed pharmacokinetic and lipidomic study on migraine model rats after administration of PI.
For the detection of the compounds in plasma, AB Sciex Triple Quad™ 4500 was applied for quantitative analysis, and the COSMOSIL C18 column (2.1 × 100 mm, 2.6 μm) was used for separation.
Isorhynchophylline (ISO: m/z 384.8–241.2) and its main metabolite rhynchophylline (RHY: m/z 384.8–160.2) were simultaneously detected under positive ion modes.
Besides, parishin A (PA: m/z 995.1–726.9) and its main metabolite gastrodin (GAS: m/z 331.1–123.0) were simultaneously detected with negative ion modes.
For the analysis of endogenous lipid components, Dionex Ultimate 3000 (UHPLC) Thermo Orbitrap Elite was applied for the detection, and the Waters UPLCRBEH C18 column (1.7 μm 100 ∗ 2.1 mm) was used for separation.
Chloroform/methanol (2 : 1, v : v) was used for extraction.
The results demonstrated that PI exists significant difference in metabolism between single- and coadministration and can regulate lipid levels associated with migraine.
American Psychological Association (APA)
Zhou, Chaoqun& He, Mingzhen& Peng, Chunyan& Ouyang, Hui& Li, Zhifeng& Zhou, Maofu…[et al.]. 2020. Pharmacokinetic and Lipidomic Assessment of the In Vivo Effects of Parishin A-Isorhynchophylline in Rat Migraine Models. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1175040
Modern Language Association (MLA)
Zhou, Chaoqun…[et al.]. Pharmacokinetic and Lipidomic Assessment of the In Vivo Effects of Parishin A-Isorhynchophylline in Rat Migraine Models. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1175040
American Medical Association (AMA)
Zhou, Chaoqun& He, Mingzhen& Peng, Chunyan& Ouyang, Hui& Li, Zhifeng& Zhou, Maofu…[et al.]. Pharmacokinetic and Lipidomic Assessment of the In Vivo Effects of Parishin A-Isorhynchophylline in Rat Migraine Models. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1175040
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1175040