Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy

Joint Authors

Hou, Xuwei
Li, Zhaohui
Higashi, Yusuke
Delafontaine, Patrice
Sukhanov, Sergiy

Source

Journal of Aging Research

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-08-01

Country of Publication

Egypt

No. of Pages

13

Abstract EN

Mitochondrial dysfunction is a hallmark of cellular aging.

Mitophagy is a critical mitochondrial quality control mechanism that removes dysfunctional mitochondria and contributes to cell survival.

Insulin-like growth factor 1 (IGF-1) promotes survival of smooth muscle cells (SMCs), but its potential effect on cellular aging is unknown yet.

We found that IGF-1 decreased cell senescence, prevented DNA telomere shortening, increased mitochondrial membrane potential, activated cytochrome C oxidase, and reduced mitochondrial DNA damage in long-term cultured (aged) aortic SMC, suggesting an antiaging effect.

IGF-1 increased mitophagy in aged cells, and this was associated with decreased expression of cyclin-dependent kinase inhibitors p16 and p21 and elevated levels of Nrf2 and Sirt3, regulators of mitophagy and mitochondrial biogenesis.

SiRNA-induced inhibition of either Nrf2 or Sirt3 blocked IGF-1-induced upregulation of mitophagy, suggesting that the Nrf2/Sirt3 pathway was required for IGF-1’s effect on mitophagy.

PINK1 is a master regulator of mitophagy.

PINK1 silencing suppressed mitophagy and inhibited IGF-1-induced antiaging effects in aged SMC, consistent with an essential role of mitophagy in IGF-1’s effect on cellular aging.

Thus, IGF-1 inhibited cellular aging via Nrf2/Sirt3-dependent activation of mitophagy.

Our data suggest that activation of IGF-1 signaling is a novel potential strategy to activate mitophagy and slow cellular aging.

American Psychological Association (APA)

Hou, Xuwei& Li, Zhaohui& Higashi, Yusuke& Delafontaine, Patrice& Sukhanov, Sergiy. 2020. Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy. Journal of Aging Research،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1175095

Modern Language Association (MLA)

Hou, Xuwei…[et al.]. Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy. Journal of Aging Research No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1175095

American Medical Association (AMA)

Hou, Xuwei& Li, Zhaohui& Higashi, Yusuke& Delafontaine, Patrice& Sukhanov, Sergiy. Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy. Journal of Aging Research. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1175095

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1175095