Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy
Joint Authors
Hou, Xuwei
Li, Zhaohui
Higashi, Yusuke
Delafontaine, Patrice
Sukhanov, Sergiy
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-01
Country of Publication
Egypt
No. of Pages
13
Abstract EN
Mitochondrial dysfunction is a hallmark of cellular aging.
Mitophagy is a critical mitochondrial quality control mechanism that removes dysfunctional mitochondria and contributes to cell survival.
Insulin-like growth factor 1 (IGF-1) promotes survival of smooth muscle cells (SMCs), but its potential effect on cellular aging is unknown yet.
We found that IGF-1 decreased cell senescence, prevented DNA telomere shortening, increased mitochondrial membrane potential, activated cytochrome C oxidase, and reduced mitochondrial DNA damage in long-term cultured (aged) aortic SMC, suggesting an antiaging effect.
IGF-1 increased mitophagy in aged cells, and this was associated with decreased expression of cyclin-dependent kinase inhibitors p16 and p21 and elevated levels of Nrf2 and Sirt3, regulators of mitophagy and mitochondrial biogenesis.
SiRNA-induced inhibition of either Nrf2 or Sirt3 blocked IGF-1-induced upregulation of mitophagy, suggesting that the Nrf2/Sirt3 pathway was required for IGF-1’s effect on mitophagy.
PINK1 is a master regulator of mitophagy.
PINK1 silencing suppressed mitophagy and inhibited IGF-1-induced antiaging effects in aged SMC, consistent with an essential role of mitophagy in IGF-1’s effect on cellular aging.
Thus, IGF-1 inhibited cellular aging via Nrf2/Sirt3-dependent activation of mitophagy.
Our data suggest that activation of IGF-1 signaling is a novel potential strategy to activate mitophagy and slow cellular aging.
American Psychological Association (APA)
Hou, Xuwei& Li, Zhaohui& Higashi, Yusuke& Delafontaine, Patrice& Sukhanov, Sergiy. 2020. Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy. Journal of Aging Research،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1175095
Modern Language Association (MLA)
Hou, Xuwei…[et al.]. Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy. Journal of Aging Research No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1175095
American Medical Association (AMA)
Hou, Xuwei& Li, Zhaohui& Higashi, Yusuke& Delafontaine, Patrice& Sukhanov, Sergiy. Insulin-Like Growth Factor I Prevents Cellular Aging via Activation of Mitophagy. Journal of Aging Research. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1175095
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1175095