Marinobufagenin Inhibits Neutrophil Migration and Proinflammatory Cytokines
Joint Authors
Cavalcante-Silva, Luiz Henrique Agra
Alves, Anne Kaliery De Abreu
Galvão, José Guilherme Marques
Carvalho, Deyse C. M.
Lima, Éssia de A.
Feijó, Priscilla R. O.
Quintas, Luís E. M.
Rodrigues-Mascarenhas, Sandra
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Cardiotonic steroids, such as ouabain and digoxin, are known to bind to Na+/K+-ATPase and to promote several biological activities, including anti-inflammatory activity.
However, there are still no reports in the literature about inflammation and marinobufagenin, a cardiotonic steroid from the bufadienolide family endogenously found in mammals.
Therefore, the aim of this work was to analyze, in vivo and in vitro, the role of marinobufagenin in acute inflammation.
Swiss mice were treated with 0.56 mg/kg of marinobufagenin intraperitoneally (i.p.) and zymosan (2 mg/mL, i.p.) was used to induce peritoneal inflammation.
Peritoneal fluid was collected and used for counting cells by optical microscopy and proinflammatory cytokine quantification (IL-1β, IL-6, and TNF-α) by immunoenzymatic assay (ELISA).
Zymosan stimulation, as expected, induced increased cell migration and proinflammatory cytokine levels in the peritoneum.
Marinobufagenin treatment reduced polymorphonuclear cell migration and IL-1β and IL-6 levels in the peritoneal cavity, without interfering in TNF-α levels.
In addition, the effect of marinobufagenin was evaluated using peritoneal macrophages stimulated by zymosan (0.2 mg/mL) in vitro.
Marinobufagenin treatment at different concentrations (10, 100, 1000, and 10000 nM) showed no cytotoxic effect on peritoneal macrophages.
Interestingly, the lowest concentration, which did not inhibit Na+/K+-ATPase activity, attenuated proinflammatory cytokines IL-1β, IL-6, and TNF-α levels.
To investigate the putative mechanism of action of marinobufagenin, the expression of surface molecules (TLR2 and CD69) and P-p38 MAPK were also evaluated, but no significant effect was observed.
Thus, our results suggest that marinobufagenin has an anti-inflammatory role in vivo and in vitro and reveals a novel possible endogenous function of this steroid in mammals.
American Psychological Association (APA)
Carvalho, Deyse C. M.& Cavalcante-Silva, Luiz Henrique Agra& Lima, Éssia de A.& Galvão, José Guilherme Marques& Alves, Anne Kaliery De Abreu& Feijó, Priscilla R. O.…[et al.]. 2019. Marinobufagenin Inhibits Neutrophil Migration and Proinflammatory Cytokines. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1175648
Modern Language Association (MLA)
Carvalho, Deyse C. M.…[et al.]. Marinobufagenin Inhibits Neutrophil Migration and Proinflammatory Cytokines. Journal of Immunology Research No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1175648
American Medical Association (AMA)
Carvalho, Deyse C. M.& Cavalcante-Silva, Luiz Henrique Agra& Lima, Éssia de A.& Galvão, José Guilherme Marques& Alves, Anne Kaliery De Abreu& Feijó, Priscilla R. O.…[et al.]. Marinobufagenin Inhibits Neutrophil Migration and Proinflammatory Cytokines. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1175648
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1175648