Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis
Joint Authors
Dias-Melicio, Luciane Alarcão
de Quaglia e Silva, Juliana Carvalho
Della Coletta, Amanda Manoel
Gardizani, Taiane Priscila
Romagnoli, Graziela Gorete
Kaneno, Ramon
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Paracoccidioidomycosis (PCM), a systemic mycosis endemic in Latin America, occurs after inhalation of mycelial components of Paracoccidioides spp.
When the fungus reaches the lungs and interacts with the alveolar macrophages and other cells, phagocytic cells such as neutrophils and monocytes are immediately recruited to the injured site.
The interaction between surface molecules of pathogens and homologous receptors, present on the surface membrane of phagocytes, modulates the innate immune cell activation.
Studies have shown the importance of fungal recognition by the Dectin-1 receptor, which can induce a series of cellular protective responses against fungi.
The objective of the present study was to evaluate Dectin-1 receptor expression and the effector mechanisms of human monocytes and neutrophils activated or not with different cytokines, such as IFN-γ, TNF-α, and GM-CSF, followed by the challenge with Paracoccidioides brasiliensis (P.
brasiliensis or Pb265).
Therefore, analysis of Dectin-1 receptor expression was done by flow cytometry whereas the effector mechanisms were evaluated by fungal recovery by colony-forming unit (CFU) counting and hydrogen peroxide (H2O2) production.
Our results showed that, after treatment with IFN-γ, TNF-α, and GM-CSF and challenge with Pb265, cells, especially monocytes, demonstrated an increase in Dectin-1 expression.
Both types of cells treated with the cytokines exhibited a decreased fungal recovery and, conversely, an increased production of H2O2.
However, when cultures were treated with an anti-Dectin-1 monoclonal antibody, to block the P.
brasiliensis binding, a decrease in H2O2 production and an increase in fungal recovery were detected.
This effect was observed in all cultures treated with the specific monoclonal antibody.
These results show the involvement of the Dectin-1 receptor in fungal recognition and its consequent participation in the induction of the killing mechanisms against P.
brasiliensis.
American Psychological Association (APA)
de Quaglia e Silva, Juliana Carvalho& Della Coletta, Amanda Manoel& Gardizani, Taiane Priscila& Romagnoli, Graziela Gorete& Kaneno, Ramon& Dias-Melicio, Luciane Alarcão. 2019. Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1175695
Modern Language Association (MLA)
de Quaglia e Silva, Juliana Carvalho…[et al.]. Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis. Journal of Immunology Research No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1175695
American Medical Association (AMA)
de Quaglia e Silva, Juliana Carvalho& Della Coletta, Amanda Manoel& Gardizani, Taiane Priscila& Romagnoli, Graziela Gorete& Kaneno, Ramon& Dias-Melicio, Luciane Alarcão. Involvement of the Dectin-1 Receptor upon the Effector Mechanisms of Human Phagocytic Cells against Paracoccidioides brasiliensis. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1175695
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1175695