Cloning, Characterization, and Functional Expression of Phospholipase Dα cDNA from Banana (Musa acuminate L.)
Joint Authors
Li, Li
Sun, Jian
He, Xuemei
Liu, Guoming
Zheng, Fengjin
Tang, Yayuan
Li, Changbao
Ling, Dongning
Sheng, Jinfeng
You, Xiangrong
Li, Jiemin
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-10-22
Country of Publication
Egypt
No. of Pages
7
Abstract EN
Phospholipase D (PLD) plays a key role in adaptive responses of postharvest fruits.
A cDNA clone of banana (Musa acuminate L.) PLDα (MaPLDα) was obtained by RT-PCR in this study.
The MaPLDα gene contains a complete open reading frame (ORF) encoding a 92-kDa protein composed of 832 amino acid residues and possesses a characteristic C2 domain and two catalytic H×K×××D (abbr.
HKD) motifs.
The two HKD motifs are separated by 341 amino acid residues in the primary structure.
Relatively higher PLD activity and expression of MaPLDα mRNA were detected in developing tissues compared to senescent or mature tissues in individual leaves, flower, stem, and fruit organs, respectively.
The expression profile of PLDα mRNA in postharvest banana fruits at different temperatures was determined, and the MaPLDα mRNA reached the highest expression peak on day 5 at 25°C and on day 7 at 12°C.
The results provide useful information for maintaining postharvest quality and extending the storage life of banana fruit.
American Psychological Association (APA)
Li, Li& He, Xuemei& Sun, Jian& Li, Changbao& Ling, Dongning& Sheng, Jinfeng…[et al.]. 2017. Cloning, Characterization, and Functional Expression of Phospholipase Dα cDNA from Banana (Musa acuminate L.). Journal of Food Quality،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1175783
Modern Language Association (MLA)
Li, Li…[et al.]. Cloning, Characterization, and Functional Expression of Phospholipase Dα cDNA from Banana (Musa acuminate L.). Journal of Food Quality No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1175783
American Medical Association (AMA)
Li, Li& He, Xuemei& Sun, Jian& Li, Changbao& Ling, Dongning& Sheng, Jinfeng…[et al.]. Cloning, Characterization, and Functional Expression of Phospholipase Dα cDNA from Banana (Musa acuminate L.). Journal of Food Quality. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1175783
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1175783
