The Nod2 Agonist Muramyl Dipeptide Cooperates with the TLR4 Agonist Lipopolysaccharide to Enhance IgG2b Production in Mouse B Cells
Joint Authors
Lee, Sang-Hoon
Park, Jong-Hwan
Park, Seok-Rae
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Many studies have shown that Toll-like receptors (TLRs) and Nod-like receptors (NLRs) were expressed in B cells and their signaling affects B cell functions.
Nonetheless, the roles played by these receptors in B cell antibody (Ab) production have not been completely elucidated.
In the present study, we examined the effect of the Nod2 agonist muramyl dipeptide (MDP) in combination with the TLR4 agonist lipopolysaccharide (LPS), a well-known B cell mitogen, on B cell viability, proliferation, and activation, and Ab production by in vitro culture of purified mouse spleen resting B cells.
MDP combined with LPS to reinforce B cell viability, proliferation, and activation.
Moreover, MDP enhanced LPS-induced IgG2b production, germline γ2b transcript (GLTγ2b) expression, and surface IgG2b expression.
In an experiment with Nod2- and TLR4-deficient mouse B cells, we observed that the combined effect of MDP and LPS is dependent on Nod2 and TLR4 receptors.
Furthermore, the combined effect on B cell viability and IgG2b switching was not observed in Rip2-deficient mouse cells.
Collectively, this study suggests that Nod2 signaling enhances TLR4-activated B cell proliferation, IgG2b switching, and IgG2b production.
American Psychological Association (APA)
Lee, Sang-Hoon& Park, Jong-Hwan& Park, Seok-Rae. 2019. The Nod2 Agonist Muramyl Dipeptide Cooperates with the TLR4 Agonist Lipopolysaccharide to Enhance IgG2b Production in Mouse B Cells. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1175901
Modern Language Association (MLA)
Lee, Sang-Hoon…[et al.]. The Nod2 Agonist Muramyl Dipeptide Cooperates with the TLR4 Agonist Lipopolysaccharide to Enhance IgG2b Production in Mouse B Cells. Journal of Immunology Research No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1175901
American Medical Association (AMA)
Lee, Sang-Hoon& Park, Jong-Hwan& Park, Seok-Rae. The Nod2 Agonist Muramyl Dipeptide Cooperates with the TLR4 Agonist Lipopolysaccharide to Enhance IgG2b Production in Mouse B Cells. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1175901
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1175901