Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

Abstract EN

The goal of this research is to develop a single-walled carbon nanotube- (SWCNT-) based biosensor to detect Staphylococcus aureus.

The specificity of 11 bacteria and polyclonal anti-Staphylococcus aureus antibodies (pAbs) was determined using an indirect ELISA.

The pAbs were immobilized onto sensor platform after the hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE).

The resistance difference (ΔR) was calculated using a potentiostat.

The bacteria detected by the biosensor were observed using a scanning electron microscope (SEM).

The optimum concentration of SWCNTs on the platform was determined to be 0.1 mg/mL.

The binding of pAbs with S.

aureus resulted in a significant increase in resistance value of the biosensor (P<0.05).

The SEM images confirmed the specific binding of S.

aureus on the biosensor.

The SWCNT-based biosensor was able to detect S.

aureus with a limit of detection (LOD) of 4 logCFU/mL.