Development of a Chemically Defined Medium for Better Yield and Purification of Enterocin Y31 from Enterococcus faecium Y31
Joint Authors
Zhang, Lanwei
Liu, Wenli
Yi, Huaxi
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-06
Country of Publication
Egypt
No. of Pages
8
Abstract EN
The macro- and micronutrients in traditional medium, such as MRS, used for cultivating lactic acid bacteria, especially for bacteriocin production, have not been defined, preventing the quantitative monitoring of metabolic flux during bacteriocin biosynthesis.
To enhance Enterocin Y31 production and simplify steps of separation and purification, we developed a simplified chemically defined medium (SDM) for the growth of Enterococcus faecium Y31 and production of its bacteriocin, Enterocin Y31.
We found that the bacterial growth was unrelated to Enterocin Y31 production in MRS; therefore, both the growth rate and the Enterocin Y31 production were set as the index for investigation.
Single omission experiments revealed that 5 g/L NaCl, five vitamins, two nucleic acid bases, MgSO4·7H2O, MnSO4·4H2O, KH2PO4, K2HPO4, CH3COONa, fourteen amino acids, and glucose were essential for the strain’s growth and Enterocin Y31 production.
Thus, a novel simplified and defined medium (SDM) was formulated with 30 components in total.
Consequently, Enterocin Y31 production yield was higher in SDM as compared to either MRS or CDM.
SDM improved the Enterocin Y31 production and simplified the steps of purification (only two steps), which has broad potential applications.
American Psychological Association (APA)
Liu, Wenli& Zhang, Lanwei& Yi, Huaxi. 2017. Development of a Chemically Defined Medium for Better Yield and Purification of Enterocin Y31 from Enterococcus faecium Y31. Journal of Food Quality،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1176155
Modern Language Association (MLA)
Liu, Wenli…[et al.]. Development of a Chemically Defined Medium for Better Yield and Purification of Enterocin Y31 from Enterococcus faecium Y31. Journal of Food Quality No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1176155
American Medical Association (AMA)
Liu, Wenli& Zhang, Lanwei& Yi, Huaxi. Development of a Chemically Defined Medium for Better Yield and Purification of Enterocin Y31 from Enterococcus faecium Y31. Journal of Food Quality. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1176155
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176155