Analysis of Toll-Like Receptors in Human Milk: Detection of Membrane-Bound and Soluble Forms
Joint Authors
Manfredi, Marcello
Cattaneo, Chiara
Caramaschi, Alice
Uga, Elena
Braghin, Michela
Cosi, Gianluca
Peila, Chiara
Strozzi, Maria C.
Sabatini, Miriam
Gazzolo, Diego
Cavaletto, Maria
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The bioactive and anti-inflammatory role of human milk components has been recognized; active milk components include soluble forms of Toll-like receptors (TLRs).
Preterm babies are more susceptible to infections and may succumb to necrotizing enterocolitis (NEC), a gastrointestinal disease which is exacerbated by an excessive inflammatory response after TLR activation.
Here, we investigated the presence of Toll-like receptors TLR1/2/4/6 in colostrum and mature milk of women who delivered before (preterm) or after (term) 37 weeks of gestational age, integrating classical immune-related techniques with proteomic LC-MS/MS analysis.
We have detected immunoreactivity for TLRs mostly in preterm samples, even for TLR1 and TLR6, until now not described in human milk.
We demonstrated the presence of only TLR2 in the milk fat globule membrane, while the immunoreactivity of TLR1/4/6 was ascribed to crossreaction with some interesting milk proteins sharing leucine-rich repeat domains.
These results will provide new insights into the definition of the role of TLRs in intestinal immune regulation of the newborns.
American Psychological Association (APA)
Cattaneo, Chiara& Caramaschi, Alice& Uga, Elena& Braghin, Michela& Cosi, Gianluca& Peila, Chiara…[et al.]. 2019. Analysis of Toll-Like Receptors in Human Milk: Detection of Membrane-Bound and Soluble Forms. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176164
Modern Language Association (MLA)
Cattaneo, Chiara…[et al.]. Analysis of Toll-Like Receptors in Human Milk: Detection of Membrane-Bound and Soluble Forms. Journal of Immunology Research No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1176164
American Medical Association (AMA)
Cattaneo, Chiara& Caramaschi, Alice& Uga, Elena& Braghin, Michela& Cosi, Gianluca& Peila, Chiara…[et al.]. Analysis of Toll-Like Receptors in Human Milk: Detection of Membrane-Bound and Soluble Forms. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176164
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176164