Evaluation of EBV- and HCMV-Specific T Cell Responses in Systemic Lupus Erythematosus (SLE) Patients Using a Normalized Enzyme-Linked Immunospot (ELISPOT) Assay
Joint Authors
Baldanti, Fausto
Cassaniti, Irene
Adzasehoun, Kodjo Messan Guy
Comolli, Giuditta
Cavagna, Lorenzo
Calarota, Sandra A.
Montecucco, Carlomaurizio
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-02-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Systemic lupus erythematosus (SLE) is an autoimmune disease with a complex etiology.
Opportunistic viral pathogens, such as human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV), are particularly relevant.
The role of the T cell response in SLE has not been deeply studied; we investigated the role of HCMV- and EBV-specific T cell responses in SLE patients also in relation to their pharmacological immunosuppressive status.
PBMCs from 70 SLE patients and 50 healthy controls were stimulated with EBV- and HCMV-specific antigens, and IFN-γ-secreting T cells were quantified.
We observed that both EBV- and HCMV-specific T cell responses were significantly lower in SLE patients compared with healthy subjects.
We reported decreased EBV- and HCMV-specific T cell responses among medium-high immunosuppressed patients compared to low immunosuppressed patients.
Immunosuppressive level could exert a role in the control of herpesviruses reactivation, even if the immunosuppressive condition of SLE remains the driving cause of skewed virus-specific T cell response.
American Psychological Association (APA)
Cassaniti, Irene& Cavagna, Lorenzo& Calarota, Sandra A.& Adzasehoun, Kodjo Messan Guy& Comolli, Giuditta& Montecucco, Carlomaurizio…[et al.]. 2019. Evaluation of EBV- and HCMV-Specific T Cell Responses in Systemic Lupus Erythematosus (SLE) Patients Using a Normalized Enzyme-Linked Immunospot (ELISPOT) Assay. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176191
Modern Language Association (MLA)
Cassaniti, Irene…[et al.]. Evaluation of EBV- and HCMV-Specific T Cell Responses in Systemic Lupus Erythematosus (SLE) Patients Using a Normalized Enzyme-Linked Immunospot (ELISPOT) Assay. Journal of Immunology Research No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1176191
American Medical Association (AMA)
Cassaniti, Irene& Cavagna, Lorenzo& Calarota, Sandra A.& Adzasehoun, Kodjo Messan Guy& Comolli, Giuditta& Montecucco, Carlomaurizio…[et al.]. Evaluation of EBV- and HCMV-Specific T Cell Responses in Systemic Lupus Erythematosus (SLE) Patients Using a Normalized Enzyme-Linked Immunospot (ELISPOT) Assay. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1176191
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176191