A Stability Indicating HPLC Assay Method for Analysis of Rivastigmine Hydrogen Tartrate in Dual-Ligand Nanoparticle Formulation Matrices and Cell Transport Medium
Joint Authors
Benson, Heather A. E.
Chen, Yan
Huda, Naz Hasan
Gauri, Bhawna
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The objective of this study was to develop and validate a method for quantitative analysis of rivastigmine hydrogen tartrate (RHT) in dual-ligand polymeric nanoparticle formulation matrices, drug release medium, and cellular transport medium.
An isocratic HPLC analysis method using a reverse phase C18 column and a simple mobile phase without buffer was developed, optimised, and fully validated.
Analyses were carried out at a flow rate of 1.5 mL/min at 50°C and monitored at 214 nm.
This HPLC method exhibited good linearity, accuracy, and selectivity.
The recovery (accuracy) of RHT from all matrices was greater than 99.2%.
The RHT peak detected in the samples of a forced degradation study, drug loading study, release study, and cellular transport study was pure and free of matrix interference.
The limit of detection (LOD) and limit of quantification (LOQ) of the assay were 60 ng/mL and 201 ng/mL, respectively.
The method was rugged with good intra- and interday precision.
This stability indicating HPLC method was selective, accurate, and precise for analysing RHT loading and its stability in nanoparticle formulation, RHT release, and cell transport medium.
American Psychological Association (APA)
Huda, Naz Hasan& Gauri, Bhawna& Benson, Heather A. E.& Chen, Yan. 2018. A Stability Indicating HPLC Assay Method for Analysis of Rivastigmine Hydrogen Tartrate in Dual-Ligand Nanoparticle Formulation Matrices and Cell Transport Medium. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1176192
Modern Language Association (MLA)
Huda, Naz Hasan…[et al.]. A Stability Indicating HPLC Assay Method for Analysis of Rivastigmine Hydrogen Tartrate in Dual-Ligand Nanoparticle Formulation Matrices and Cell Transport Medium. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1176192
American Medical Association (AMA)
Huda, Naz Hasan& Gauri, Bhawna& Benson, Heather A. E.& Chen, Yan. A Stability Indicating HPLC Assay Method for Analysis of Rivastigmine Hydrogen Tartrate in Dual-Ligand Nanoparticle Formulation Matrices and Cell Transport Medium. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1176192
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176192