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Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures
Joint Authors
Liu, Jinhua
Shi, Yanyu
Teng, Siying
Wu, Lianpeng
Zhang, Xinmin
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-10
Country of Publication
Egypt
No. of Pages
8
Abstract EN
Raccoon dog (Nyctereutes procyonoides) is an economically important animal used for fur production, but consuming its meat is injurious to human health.
Currently, no rapid and sensitive method for detecting raccoon dog meat in meat mixtures is available.
In this study, we developed an easily applicable, rapid, and economically feasible method for identifying the presence of raccoon dog in meat mixtures based on loop-mediated isothermal amplification (LAMP).
Four sets of LAMP primers were tested at different temperatures, and the primers that worked best at 62°C (set 2) were determined.
In the LAMP assay, there was no cross-reactivity with the meat procured from other species of animals and the detection limit of DNA concentration was 0.1 pg·μL−1, slightly higher than TaqMan real-time PCR (0.01 pg·μL−1), but sensitivity of 0.1 pg·μL−1 complies with most requirements of routine analysis.
Moreover, by the LAMP method, the meat mixtures containing more than 0.5% of the raccoon dog component were directly detected (without DNA extraction) in the supernatant isolated from the meat mixtures after performing repeated cycles of thawing and freezing of minced meat mixtures.
Our results show that LAMP assay is a valuable, straightforward, and sensitive detection tool for identification of raccoon dog meat in mixtures.
American Psychological Association (APA)
Liu, Jinhua& Shi, Yanyu& Teng, Siying& Wu, Lianpeng& Zhang, Xinmin. 2017. Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures. Journal of Food Quality،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1176195
Modern Language Association (MLA)
Liu, Jinhua…[et al.]. Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures. Journal of Food Quality No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1176195
American Medical Association (AMA)
Liu, Jinhua& Shi, Yanyu& Teng, Siying& Wu, Lianpeng& Zhang, Xinmin. Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures. Journal of Food Quality. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1176195
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176195