A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells
Joint Authors
Liu, Lin
Ma, Xiaohua
Wu, Guoguang
Zhao, Yuehua
Yuan, Zibo
Zhang, Yu
Xia, Ning
Yang, Mengnan
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-12-13
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative.
The structure of the obtained probe was confirmed by NMR and HRMS techniques.
The probe shows a remarkable fluorescence off-on response (∼52-fold) by the reaction with Cys in 100% aqueous buffer.
The sensing mechanism was verified by the HPLC test.
Probe 1 also displays high selectivity towards Cys.
The detection limit was calculated to be 23 nM.
Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys.
American Psychological Association (APA)
Ma, Xiaohua& Wu, Guoguang& Zhao, Yuehua& Yuan, Zibo& Zhang, Yu& Xia, Ning…[et al.]. 2018. A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1176209
Modern Language Association (MLA)
Ma, Xiaohua…[et al.]. A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1176209
American Medical Association (AMA)
Ma, Xiaohua& Wu, Guoguang& Zhao, Yuehua& Yuan, Zibo& Zhang, Yu& Xia, Ning…[et al.]. A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1176209
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176209
