Screening and Characterizing Tyrosinase Inhibitors from Salvia miltiorrhiza and Carthamus tinctorius by Spectrum-Effect Relationship Analysis and Molecular Docking
Joint Authors
Hu, Yuanjia
Zhang, Qian
Wang, Ya-Li
Hu, Guang
Yang, Yu-Xiu
Li, Qiao-Qiao
Chen, Hua
Yang, F. Q.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Tyrosinase (TYR) is a rate-limiting enzyme in the synthesis of melanin, while direct TYR inhibitors are a class of important clinical antimelanoma drugs.
This study established a spectrum-effect relationship analysis method and high-performance liquid chromatography-mass spectrometry (LC-MS) analysis method to screen and identify the active ingredients that inhibited TYR in Salvia miltiorrhiza–Carthamus tinctorius (Danshen–Honghua, DH) herbal pair.
Seventeen potential active compounds (peaks) in the extract of DH herbal pair were predicted, and thirteen of them were tentatively identified by LC-MS analysis.
Furthermore, TYR inhibitory activities of five pure compounds obtained from the DH herbal pair were validated in the test in which kojic acid served as a positive control drug.
Among them, three compounds including protocatechuic aldehyde, hydroxysafflor yellow A, and tanshinone IIA were verified to have high TYR inhibitory activity (IC50 value of 455, 498, and 1214 μM, resp.) and bind to the same amino acid residues in TYR catalytic pocket according to the results of the molecular docking test.
However, the other two compounds lithospermic acid and salvianolic acid A had a weak effect on TYR, as they do not combine with the active amino acid residues or act on the active center of TYR.
Therefore, the developed methods (spectrum-effect relationship analysis and molecular docking) could be used to effectively screen TYR inhibitors in complex mixtures such as natural products.
American Psychological Association (APA)
Wang, Ya-Li& Hu, Guang& Zhang, Qian& Yang, Yu-Xiu& Li, Qiao-Qiao& Hu, Yuanjia…[et al.]. 2018. Screening and Characterizing Tyrosinase Inhibitors from Salvia miltiorrhiza and Carthamus tinctorius by Spectrum-Effect Relationship Analysis and Molecular Docking. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1176221
Modern Language Association (MLA)
Wang, Ya-Li…[et al.]. Screening and Characterizing Tyrosinase Inhibitors from Salvia miltiorrhiza and Carthamus tinctorius by Spectrum-Effect Relationship Analysis and Molecular Docking. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1176221
American Medical Association (AMA)
Wang, Ya-Li& Hu, Guang& Zhang, Qian& Yang, Yu-Xiu& Li, Qiao-Qiao& Hu, Yuanjia…[et al.]. Screening and Characterizing Tyrosinase Inhibitors from Salvia miltiorrhiza and Carthamus tinctorius by Spectrum-Effect Relationship Analysis and Molecular Docking. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1176221
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176221