Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material
Joint Authors
Maljurić, Nevena
Golubović, Jelena
Ravnikar, Matjaž
Štrukelj, Borut
Otašević, Biljana
Žigon, Dušan
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Diabetes mellitus is one of the leading world’s public health problems.
Therefore, it is of a huge interest to develop new antidiabetic drugs.
Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential.
Fomes fomentarius is a mushroom widely used for different purposes, due to its range of already confirmed activities.
Fomentariol is a constituent of Fomes fomentarius, responsible for its antidiabetic potential.
In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient.
Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction.
The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry.
Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active compound fomentariol.
It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes.
The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard.
The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol.
American Psychological Association (APA)
Maljurić, Nevena& Golubović, Jelena& Ravnikar, Matjaž& Žigon, Dušan& Štrukelj, Borut& Otašević, Biljana. 2018. Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176257
Modern Language Association (MLA)
Maljurić, Nevena…[et al.]. Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1176257
American Medical Association (AMA)
Maljurić, Nevena& Golubović, Jelena& Ravnikar, Matjaž& Žigon, Dušan& Štrukelj, Borut& Otašević, Biljana. Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176257
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176257