Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD
Joint Authors
Jeong, Ji Hyun
Lee, Seon Yu
Kim, Bo Na
Lee, Guk Yeo
Ham, Seong Ho
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-05-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
So-Cheong-Ryong-Tang, which is a standardized Korean medicine of the National Health Insurance, is a traditional prescription for the treatment of allergic rhinitis, bronchitis, and bronchial asthma.
Simultaneous analysis and development of SCRT is essential for its stability, efficacy, and risk management.
In this study, a simple, reliable, and accurate method using ultrahigh-performance liquid chromatography (UPLC) fingerprinting with a diode array detector (DAD) was developed for the simultaneous analysis.
The chromatographic separation of the analytes was performed by an ACQUITY UPLC BEH C18 column (1.7 μM, 2.1 × 100 mm, Waters) with a mobile phase of water containing 0.01% (v/v) phosphoric acid and acetonitrile containing 0.01% (v/v) phosphoric acid.
The flow rate and detection wavelength were set at 0.4 mL/min and 215, 230, 254, and 280 nm.
All calibration curves of the thirteen components showed good linearity (R2 > 0.999).
The limit of detection and limit of quantification ranged 0.001–0.360 and 0.004–1.200 µg/mL, respectively.
The relative standard deviation (RSD) of intra- and interday was less than 2.60%, and the recoveries were within the range 76.08–103.79% with an RSD value of 0.03–1.50%.
The results showed that the developed method was simple, reliable, accurate, sensitive, and precise for the quantification of bioactive components of SCRT.
American Psychological Association (APA)
Jeong, Ji Hyun& Lee, Seon Yu& Kim, Bo Na& Lee, Guk Yeo& Ham, Seong Ho. 2018. Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1176296
Modern Language Association (MLA)
Jeong, Ji Hyun…[et al.]. Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1176296
American Medical Association (AMA)
Jeong, Ji Hyun& Lee, Seon Yu& Kim, Bo Na& Lee, Guk Yeo& Ham, Seong Ho. Development of Simultaneous Analysis of Thirteen Bioactive Compounds in So-Cheong-Ryong-Tang Using UPLC-DAD. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1176296
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176296