Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities
Joint Authors
Fang, Lei
Zhang, Hua
Zhou, Jie
Geng, Yanling
Wang, Xiao
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
An efficient method using high-speed counter-current chromatography (HSCCC) coupled with DPPH-HPLC assay has been developed for rapid screening and preparative isolation of antioxidants from ethyl acetate fraction of Alpinia officinarum Hance.
Target-guided by DPPH-HPLC assay, two antioxidants, galangin and kaempferide, were targeted and further separated with purities of 99.3% and 98.5% by HSCCC using petroleum ether–ethyl acetate–methanol–water (0.8 : 1 : 1 : 0.8, v/v) as the solvent system.
The antioxidant activities of galangin and kaempferide were further evaluated by measuring their inhibiting effects on superoxide anion radical, hydroxyl radical, and hydrogen peroxide in different luminol chemiluminescence (CL) systems.
As a result, galangin and kaempferide both showed potent antioxidant activities.
Results of the present study indicated that the combinative method by offline coupling DPPH-HPLC and HSCCC could be widely applied for rapid screening and isolation of antioxidants from complex TCM extract.
American Psychological Association (APA)
Fang, Lei& Zhang, Hua& Zhou, Jie& Geng, Yanling& Wang, Xiao. 2018. Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1176298
Modern Language Association (MLA)
Fang, Lei…[et al.]. Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1176298
American Medical Association (AMA)
Fang, Lei& Zhang, Hua& Zhou, Jie& Geng, Yanling& Wang, Xiao. Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1176298
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176298