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Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry
Joint Authors
Freitas, P. V.
da Silva, D. R.
Beluomini, M. A.
da Silva, J. L.
Stradiotto, N. R.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A reversed-phase liquid chromatographic separation with pulsed amperometric detection of phenolic acids at a glassy carbon electrode is described.
Chromatographic separation was carried out in isocratic conditions using 0.20 mol·L−1 acetic acid (pH 5.0)/water (80 : 20, v/v) as mobile phase under constant working potential mode of 0.80 V.
Chromatographic peaks presented high resolution and separation.
Calibration curves exhibited excellent correlation coefficients, above 0.995.
Linear ranges of the analytes, in mg L−1, were of 0.018–18 (gallic acid), 0.146–19 (vanillic acid), 0.13–17 (caffeic acid), 0.016–16 (ferulic acid), and 0.008–17 (p-coumaric acid), respectively.
Limits of detection ranged from 1.6 to 97 μg·L−1 and precision varied in 1.73–3.78% interval.
Concentrations of 19 ± 0.51 mg·L−1 and 7.8 ± 2.5 mg·L−1 were found for vanillic and caffeic acids, respectively, in a sugarcane vinasse sample.
Gallic, ferulic, and p-coumaric acids were not detected.
Recovery results demonstrated that the proposed method is accurate, and it can be used to detect and quantify phenolic acids in sugarcane vinasse without any influence of interferents.
American Psychological Association (APA)
Freitas, P. V.& da Silva, D. R.& Beluomini, M. A.& da Silva, J. L.& Stradiotto, N. R.. 2018. Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1176380
Modern Language Association (MLA)
Freitas, P. V.…[et al.]. Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1176380
American Medical Association (AMA)
Freitas, P. V.& da Silva, D. R.& Beluomini, M. A.& da Silva, J. L.& Stradiotto, N. R.. Determination of Phenolic Acids in Sugarcane Vinasse by HPLC with Pulse Amperometry. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1176380
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176380