MTOR-Mediated Autophagy Is Involved in the Protective Effect of Ketamine on Allergic Airway Inflammation
Joint Authors
Liu, Rongyu
Zou, Hongyun
Wang, Li-Xia
Wang, Muzi
Cheng, Cheng
Li, Shuai
Shen, Qiying
Fang, Lei
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-01-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Unresolved inflammation underpins the pathogenesis of allergic airway diseases, such as asthma.
Ketamine, accepted as a promising therapy for resistant asthma, has been demonstrated to attenuate allergic airway inflammation.
However, the anti-inflammatory mechanism by ketamine in this setting is largely unknown.
We aimed to investigate whether autophagy was involved in the protective effect of ketamine on allergic airway inflammation.
Female C57BL/6 mice were sensitized to ovalbumin (OVA) and treated with ketamine at 25, 50, or 100 mg/kg prior to OVA challenge.
In this model, the pulmonary morphological findings and airway inflammation were significantly inhibited at 50 mg/kg but not at 25 or 100 mg/kg.
Moreover, 50 mg/kg ketamine abrogated the increased concentrations of inflammatory cytokines in bronchoalveolar lavage fluid (BALF) of allergic mice, as well as activated the expression of phosphorylated mammalian target of rapamycin (p-MTOR) and inhibited autophagy in allergic mice.
To confirm whether the effect of 50 mg/kg ketamine on asthma was mediated by inhibiting autophagy, rapamycin was administered to mice sensitized to OVA and exposed to 50 mg/kg ketamine.
All of the effect of 50 mg/kg ketamine was reversed by rapamycin treatment, including increased p-MTOR and decreased autophagy.
Taken together, the present study demonstrates that 50 mg/kg ketamine inhibits allergic airway inflammation by suppressed autophagy, and this effect is mediated by the activation of MTOR in the lungs of allergic mice.
American Psychological Association (APA)
Zou, Hongyun& Wang, Li-Xia& Wang, Muzi& Cheng, Cheng& Li, Shuai& Shen, Qiying…[et al.]. 2019. MTOR-Mediated Autophagy Is Involved in the Protective Effect of Ketamine on Allergic Airway Inflammation. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1176384
Modern Language Association (MLA)
Zou, Hongyun…[et al.]. MTOR-Mediated Autophagy Is Involved in the Protective Effect of Ketamine on Allergic Airway Inflammation. Journal of Immunology Research No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1176384
American Medical Association (AMA)
Zou, Hongyun& Wang, Li-Xia& Wang, Muzi& Cheng, Cheng& Li, Shuai& Shen, Qiying…[et al.]. MTOR-Mediated Autophagy Is Involved in the Protective Effect of Ketamine on Allergic Airway Inflammation. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1176384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176384