CD33 (Siglec-3) Inhibitory Function: Role in the NKG2DDAP10 Activating Pathway
Joint Authors
Ruiz-Alcaraz, Antonio José
García-Peñarrubia, Pilar
Hernández-Caselles, Trinidad
Miguel, Rubén Corral-San
Source
Journal of Immunology Research
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-04-15
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells.
It may constitute a control mechanism of the innate immune system; nevertheless, its role as an inhibitory receptor remains elusive.
Using human NK cells as a cellular model, we analyzed CD33 inhibitory function upon different activating receptors.
In high-cytotoxicity NKL cells, CD33 displayed a prominent inhibition on cytotoxicity triggered by the activating receptors NKG2D and, in a lower extent, 2B4, whereas it did not inhibit NKp46-induced cytotoxicity.
NKp46 was partially inhibited by CD33 only when low-cytotoxicity NKL cells were tested.
CD33 triggering did not inhibit IFN-γ secretion, contrasting with ILT-2 and CD94/NKG2A inhibitory receptors that inhibited cytotoxicity and IFN-γ secretion induced by all activating receptors tested.
CD33-mediated inhibition of NKG2D-induced triggering involved Vav1 dephosphorylation.
Our results support the role of CD33 as an inhibitory receptor preferentially regulating the NKG2D/DAP10 cytotoxic signaling pathway, which could be involved in self-tolerance and tumor and infected cell recognition.
American Psychological Association (APA)
Hernández-Caselles, Trinidad& Miguel, Rubén Corral-San& Ruiz-Alcaraz, Antonio José& García-Peñarrubia, Pilar. 2019. CD33 (Siglec-3) Inhibitory Function: Role in the NKG2DDAP10 Activating Pathway. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1176404
Modern Language Association (MLA)
Hernández-Caselles, Trinidad…[et al.]. CD33 (Siglec-3) Inhibitory Function: Role in the NKG2DDAP10 Activating Pathway. Journal of Immunology Research No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1176404
American Medical Association (AMA)
Hernández-Caselles, Trinidad& Miguel, Rubén Corral-San& Ruiz-Alcaraz, Antonio José& García-Peñarrubia, Pilar. CD33 (Siglec-3) Inhibitory Function: Role in the NKG2DDAP10 Activating Pathway. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1176404
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176404