Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product
Joint Authors
Zaghary, Wafaa A.
Mowaka, Shereen
Ayoub, Bassam M.
Hassan, Mostafa A.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
New spectrophotometric and chemometric methods were carried out for the simultaneous assay of trelagliptin (TRG) and its acid degradation product (TAD) and applied successfully as a stability indicating assay to recently approved Zafatek® tablets.
TAD was monitored using TLC to ensure complete degradation.
Furthermore, HPLC was used to confirm dealing with one major acid degradation product.
The proposed methods were developed by manipulating zero-order, first-derivative, and ratio spectra of TRG and TAD using simultaneous equation, first-derivative, and mean-centering methods, respectively.
Using Spectra Manager II and Minitab v.14 software, the absorbance at 274 nm–260.4 nm, amplitudes at 260.4 nm–274.0 nm, and mean-centered values at 287.6 nm–257.2 nm were measured against methanol as a blank for TRG and TAD, respectively.
Linearity and the other validation parameters were acceptable at concentration ranges of 5–50 μg/mL and 2.5–25 μg/mL for TRG and TAD, respectively.
Using one-way analysis of variance (ANOVA), the optimized methods were compared and proved to be accurate for the simultaneous assay of TRG and TAD.
American Psychological Association (APA)
Mowaka, Shereen& Ayoub, Bassam M.& Hassan, Mostafa A.& Zaghary, Wafaa A.. 2018. Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176489
Modern Language Association (MLA)
Mowaka, Shereen…[et al.]. Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1176489
American Medical Association (AMA)
Mowaka, Shereen& Ayoub, Bassam M.& Hassan, Mostafa A.& Zaghary, Wafaa A.. Different Spectrophotometric Methods for Simultaneous Determination of Trelagliptin and Its Acid Degradation Product. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1176489
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176489